Morphological Regulation of Rabbit Chondrocytes on Glucose-displayed Surface.
From: Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
Biomaterials
- Publish Date: Mar 2007
- ISSN: 0142-9612
- Volume: 28
- Issue: 9
- Pages: 1680-8
- Medium: Print
- Language: English
- Citation (JAMA): Kino-oka Masahiro, Morinaga Yoshiki, Kim Mee-Hae, et al. Morphological Regulation of Rabbit Chondrocytes on Glucose-displayed Surface.. Biomaterials Mar 2007;28:1680-8
Abstract
A culture surface was designed to regulate morphology of rabbit chondrocytes by changing the ratio of D- and L-glucose isomers displayed on a glass plate. With increasing ratio of d-glucose displayed on the surfaces, the efficiency of cell attachment improved, meaning that the attachment exclusively occurred via mediation of an affinity between D-glucose displayed and glucose transporter on cell membrane. At 0% and 100% D-glucose display, the round-shaped cells appeared dominantly, and most of cells became stretched in shape at 50% d-glucose display, indicating that the frequency of round-shaped cells depicted a concave profile against the ratio of D-glucose displayed. From the cytoskeletal staining of F-actin and vinculin, the immature stress fibers with fewer focal contacts were recognized in both the round shaped cells and those stretched in shape on 100% D-glucose-displayed surface. The time-lapse observation revealed that the cells on 100% D-glucose-displayed surface conducted active migration and aggregation with formation of collagen type II. These results suggest that 100% D-glucose-displayed surface can offer culture environment to maintain the chondrocytic phenotype of cells, similarly to the conditions achieved in three-dimensional (3-D) culture.
Mesh Headings (Keywords): Animals, Cell Adhesion, Cell Culture Techniques, Cell Differentiation, Cell Proliferation, Cells, Cultured, Chondrocytes, Chondrogenesis, Coated Materials, Biocompatible, Glucose, Materials Testing, Rabbits, Tissue Engineering
Check for Full Text / PubMed Unique Identifier (PMID): 17182094
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