Transcription Factors Involved in the Expression of Slc28 Genes in Human Liver Parenchymal Cells.
From: Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, and Institut de Biomedicina de la Universitat de Barcelona (IBUB), Diagonal 645, E-08028 Barcelona, Spain.
Biochemical and biophysical research communications
- Publish Date: Feb 2007
- ISSN: 0006-291X
- Volume: 353
- Issue: 2
- Pages: 381-8
- Medium: Print
- Language: English
- Citation (JAMA): Fernández-Veledo Sonia, Jover Ramiro, Casado F Javier, et al. Transcription Factors Involved in the Expression of Slc28 Genes in Human Liver Parenchymal Cells.. Biochem. Biophys. Res. Commun. Feb 2007;353:381-8
Abstract
Human nucleoside transporters are encoded by SLC28 (hCNTs) and SLC29 (hENTs) genes. These proteins mediate the uptake of anticancer and some antiviral drugs and are also suitable candidates to facilitate nucleoside-derived drug uptake into hepatocytes for detoxification. Despite the putative relevance of these genes in liver physiology, the human SLC28 and SLC29 expression pattern is not known and suitable cell models are not available. These issues have been addressed by examining NT expression in human liver and primary cultures of human hepatocytes. Moreover, the effect of specific liver enriched transcription factors (LETFs) in hCNTs expression has been analyzed. Human hepatocytes express hCNT1, hCNT2, hENT1, and hENT2. Loss of the hepatic phenotype in primary culture is associated with a decrease in hCNT1 and hCNT2 mRNA levels. Selected LETFs are involved in the regulation of SLC28 genes in an isoform-specific manner. HNF4alpha is a major determinant of SLC28A1 expression, whereas C/EBPalpha and HNF3gamma modulate SLC28A2 gene expression.
Mesh Headings (Keywords): Cells, Cultured, Gene Expression, Hepatocytes, Humans, Membrane Transport Proteins, Trans-Activation (Genetics), Transcription Factors
Check for Full Text / PubMed Unique Identifier (PMID): 17187757
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.