Danggui Buxue Tang--a Chinese Herbal Decoction Activates the Phosphorylations of Extracellular Signal-regulated Kinase and Estrogen Receptor Alpha in Cultured Mcf-7 Cells.
From: Department of Biology and Center for Chinese Medicine R&D, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, China.
FEBS letters
- Publish Date: Jan 2007
- ISSN: 0014-5793
- Volume: 581
- Issue: 2
- Pages: 233-40
- Medium: Print
- Language: English
- Citation (JAMA): Gao Qiu T, Choi Roy C Y, Cheung Anna W H, et al. Danggui Buxue Tang--a Chinese Herbal Decoction Activates the Phosphorylations of Extracellular Signal-regulated Kinase and Estrogen Receptor Alpha in Cultured Mcf-7 Cells.. FEBS Lett. Jan 2007;581:233-40
Abstract
Danggui buxue tang (DBT), a Chinese herbal decoction used to treat ailments in women, contains radix Astragali (Huangqi; RA) and radix Angelicae Sinensis (Danggui; RAS). The weight ratio of RA to RAS used in DBT must be 5:1 as stipulated as early as AD 1247; however, DBT’s mechanism of action has never been described. Here, the estrogenic effects of DBT were investigated by determining the phosphorylations of estrogen receptor alpha (ER alpha) and extracellular signal-regulated kinase 1/2 (Erk1/2) in cultured MCF-7 cells. The application of DBT triggered the phosphorylation of ER alpha and Erk1/2 in a time-dependent manner. In contrast to the effect of estrogen, DBT triggered ER alpha phosphorylation at both S118 and S167. This DBT-specific phosphorylation was not triggered by an extract of one of the individual herbs, or by mixing the extracts of RA and RAS. DBT-induced downstream signals are described here. These signals suggest the uniqueness of this Chinese herbal decoction that requires a well-defined formulation.
Mesh Headings (Keywords): Cells, Cultured, Drugs, Chinese Herbal, Estrogen Receptor alpha, Extracellular Signal-Regulated MAP Kinases, Humans, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Phosphorylation, Phytoestrogens, Plant Extracts, Tumor Cells, Cultured
Check for Full Text / PubMed Unique Identifier (PMID): 17187784
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