Redesigning Protein Pka Values.
From: School of Biomolecular and Biomedical Science, Centre for Synthesis and Chemical Biology, UCD Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland.
Protein science : a publication of the Protein Society
- Publish Date: Feb 2007
- ISSN: 0961-8368
- Volume: 16
- Issue: 2
- Pages: 239-49
- Medium: Print
- Language: English
- Citation (JAMA): Tynan-Connolly Barbara Mary, Nielsen Jens Erik, et al. Redesigning Protein Pka Values.. Protein Sci. Feb 2007;16:239-49
Abstract
The ability to re-engineer enzymatic pH-activity profiles is of importance for industrial applications of enzymes. We theoretically explore the feasibility of re-engineering enzymatic pH-activity profiles by changing active site pK(a) values using point mutations. We calculate the maximum achievable DeltapK(a) values for 141 target titratable groups in seven enzymes by introducing conservative net-charge altering point mutations. We examine the importance of the number of mutations introduced, their distance from the target titratable group, and the characteristics of the target group itself. The results show that multiple mutations at 10A can change pK(a) values up to two units, but that the introduction of a requirement to keep other pK(a) values constant reduces the magnitude of the achievable DeltapK(a). The algorithm presented shows a good correlation with existing experimental data and is available for download and via a web server at http://enzyme.ucd.ie/pKD.
Mesh Headings (Keywords): Algorithms, Binding Sites, Computational Biology, Electrostatics, Hydrogen-Ion Concentration, Kinetics, Mutation, Proteins, Reproducibility of Results
Check for Full Text / PubMed Unique Identifier (PMID): 17189477
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