Quantitative Proteomic Profiling of Membrane Proteins from the Mouse Brain Cortex, Hippocampus, and Cerebellum Using the Hystag Reagent: Mapping of Neurotransmitter Receptors and Ion Channels.
From: Center for Experimental BioInformatics (CEBI), University of Southern Denmark, Denmark.
Brain research
- Publish Date: Feb 2007
- ISSN: 0006-8993
- Volume: 1134
- Issue: 1
- Pages: 95-106
- Medium: Print
- Language: English
- Citation (JAMA): Olsen Jesper V, Nielsen Peter Aa, Andersen Jens R, et al. Quantitative Proteomic Profiling of Membrane Proteins from the Mouse Brain Cortex, Hippocampus, and Cerebellum Using the Hystag Reagent: Mapping of Neurotransmitter Receptors and Ion Channels.. Brain Res. Feb 2007;1134:95-106
Abstract
Analysis of the brain proteome and studying brain diseases through clinical biopsies and animal disease models require methods of quantitative proteomics that are sensitive and allow identification and quantification of low abundant membrane proteins from minute amount of tissue. Taking advantage of recently developed methods for isolation of membrane proteins from 10-20 mg brain tissue [Nielsen, P.Aa., Olsen, J.V., Podtelejnokov, A.V., Andersen, J.R., Mann, M., Wiśniewski, J.R., 2005. Proteomic mapping of brain plasma membrane proteins. Mol. Cell. Proteomics 4, 402 — 408] and the HysTag-quantification method [Olsen, J.V., Andersen, J.R., Nielsen, P.Aa., Nielsen, M.L., Figeys, D., Mann, M., Wiśniewski, J.R., 2004. HysTag — -A novel proteomic qualification tool applied to differential analysis of membrane proteins from distinct areas of mouse brain. Mol. Cell. Proteomics 3, 82 — 92] we performed quantitative proteomic analysis of three functionally distinct compartments of mouse brain: cortex, hippocampus, and cerebellum. In total, 976 unique peptides corresponding to 555 unique proteins were quantified. Up to 20-fold differences in the levels of some proteins between brain areas were measured. For many quantified proteins — as for glutamate receptors, calcium channel subunits, and ATP-ases — an excellent correlation between our proteomic data and previously published mRNA expression levels or intensity of immunostaining was found. Our results clearly demonstrate differences in levels of membrane proteins mapped in distinct brain compartments and offer a technology that allows in depth study of brain membrane proteomes, such as mouse models of neurological diseases.
Mesh Headings (Keywords): Animals, Cerebellum, Cerebral Cortex, Female, Hippocampus, Indicators and Reagents, Ion Channels, Membrane Proteins, Mice, Mice, Inbred C57BL, Proteomics, Receptors, Neurotransmitter, Subcellular Fractions
Check for Full Text / PubMed Unique Identifier (PMID): 17207779
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