Solution Nmr of Large Molecules and Assemblies.
From: Department of Biochemistry, The Ohio State University, 484 West 12th Avenue, Columbus, Ohio 43210, USA. foster.281@osu.edu
Biochemistry
- Publish Date: Jan 2007
- ISSN: 0006-2960
- Volume: 46
- Issue: 2
- Pages: 331-40
- Medium: Print
- Language: English
- Citation (JAMA): Foster Mark P, McElroy Craig A, Amero Carlos D, et al. Solution Nmr of Large Molecules and Assemblies.. Biochemistry Jan 2007;46:331-40
Abstract
Solution NMR spectroscopy represents a powerful tool for examining the structure and function of biological macromolecules. The advent of multidimensional (2D-4D) NMR, together with the widespread use of uniform isotopic labeling of proteins and RNA with the NMR-active isotopes, 15N and 13C, opened the door to detailed analyses of macromolecular structure, dynamics, and interactions of smaller macromolecules (< approximately 25 kDa). Over the past 10 years, advances in NMR and isotope labeling methods have expanded the range of NMR-tractable targets by at least an order of magnitude. Here we briefly describe the methodological advances that allow NMR spectroscopy of large macromolecules and their complexes and provide a perspective on the wide range of applications of NMR to biochemical problems.
Mesh Headings (Keywords): Binding Sites, Macromolecular Substances, Models, Molecular, Multiprotein Complexes, Nuclear Magnetic Resonance, Biomolecular, Nucleic Acid Conformation, Protein Conformation, Proteins, RNA, Solutions, Thermodynamics
Check for Full Text / PubMed Unique Identifier (PMID): 17209543
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