Expression Patterns of H2-o in Mouse B Cells and Dendritic Cells Correlate with Cell Function.
From: Cell Biology and Genetics Program, Weill Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA.
Journal of immunology (Baltimore, Md. : 1950)
- Publish Date: Feb 2007
- ISSN: 0022-1767
- Volume: 178
- Issue: 3
- Pages: 1488-97
- Medium: Print
- Language: English
- Citation (JAMA): Fallas Jennifer L, Yi Woelsung, Draghi Nicole A, et al. Expression Patterns of H2-o in Mouse B Cells and Dendritic Cells Correlate with Cell Function.. J. Immunol. Feb 2007;178:1488-97
Abstract
In the endosomes of APCs, the MHC class II-like molecule H2-M catalyzes the exchange of class II-associated invariant chain peptides (CLIP) for antigenic peptides. H2-O is another class II-like molecule that modulates the peptide exchange activity of H2-M. Although the expression pattern of H2-O in mice has not been fully evaluated, H2-O is expressed by thymic epithelial cells, B cells, and dendritic cells (DCs). In this study, we investigated H2-O, H2-M, and I-A(b)-CLIP expression patterns in B cell subsets during B cell development and activation. H2-O was first detected in the transitional 1 B cell subset and high levels were maintained in marginal zone and follicular B cells. H2-O levels were down-regulated specifically in germinal center B cells. Unexpectedly, we found that mouse B cells may have a pool of H2-O that is not associated with H2-M. Additionally, we further evaluate H2-O and H2-M interactions in mouse DCs, as well as H2-O expression in bone marrow-derived DCs. We also evaluated H2-O, H2-M, I-A(b), and I-A(b)-CLIP expression in splenic DC subsets, in which H2-O expression levels varied among the splenic DC subsets. Although it has previously been shown that H2-O modifies the peptide repertoire, H2-O expression did not alter DC presentation of a number of endogenous and exogenous Ags. Our further characterization of H2-O expression in DCs, as well as the identification of a potential free pool of H2-O in mouse splenic B cells, suggest that H2-O may have a yet to be elucidated role in immune responses.
Mesh Headings (Keywords): Animals, Antigen Presentation, B-Lymphocytes, Dendritic Cells, Gene Expression Regulation, Histocompatibility Antigens Class II, Mice, Mice, Inbred C57BL, Mice, Knockout, Spleen
Check for Full Text / PubMed Unique Identifier (PMID): 17237397
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