High Concentration of Antioxidants N-acetylcysteine and Mitoquinone-q Induces Intercellular Adhesion Molecule 1 and Oxidative Stress by Increasing Intracellular Glutathione.
From: Department of Internal Medicine, Pulmonary Division, University of Utah Health Science Center, Salt Lake City, UT 84112, USA. tapan.mukherjee@hsc.utah.edu
Journal of immunology (Baltimore, Md. : 1950)
- Publish Date: Feb 2007
- ISSN: 0022-1767
- Volume: 178
- Issue: 3
- Pages: 1835-44
- Medium: Print
- Language: English
- Citation (JAMA): Mukherjee Tapan K, Mishra Anurag K, Mukhopadhyay Srirupa, et al. High Concentration of Antioxidants N-acetylcysteine and Mitoquinone-q Induces Intercellular Adhesion Molecule 1 and Oxidative Stress by Increasing Intracellular Glutathione.. J. Immunol. Feb 2007;178:1835-44
Abstract
In endothelial cells, the intracellular level of glutathione is depleted during offering protection against proinflammatory cytokine TNF-alpha-induced oxidative stress. Administration of anti-inflammatory drugs, i.e., N-acetylcysteine (NAC) or mitoquinone-Q (mito-Q) in low concentrations in the human pulmonary aortic endothelial cells offered protection against depletion of reduced glutathione and oxidative stress mediated by TNF-alpha. However, this study addressed that administration of NAC or mito-Q in high concentrations resulted in a biphasic response by initiating an enhanced generation of both reduced glutathione and oxidized glutathione and enhanced production of reactive oxygen species, along with carbonylation and glutathionylation of the cellular proteins. This study further addressed that IkappaB kinase (IKK), a phosphorylation-dependent regulator of NF-kappaB, plays an important regulatory role in the TNF-alpha-mediated induction of the inflammatory cell surface molecule ICAM-1. Of the two catalytic subunits of IKK (IKKalpha and IKKbeta), low concentrations of NAC and mito-Q activated IKKalpha activity, thereby inhibiting the downstream NF-kappaB and ICAM-1 induction by TNF-alpha. High concentrations of NAC and mito-Q instead caused glutathionylation of IKKalpha, thereby inhibiting its activity that in turn enhanced the downstream NF-kappaB activation and ICAM-1 expression by TNF-alpha. Thus, establishing IKKalpha as an anti-inflammatory molecule in endothelial cells is another focus of this study. This is the first report that describes a stressful situation in the endothelial cells created by excess of antioxidative and anti-inflammatory agents NAC and mito-Q, resulting in the generation of reactive oxygen species, carbonylation and glutathionylation of cellular proteins, inhibition of IKKalpha activity, and up-regulation of ICAM-1expression.
Mesh Headings (Keywords): Acetylcysteine, Anti-Inflammatory Agents, Antioxidants, Aorta, Cells, Cultured, Endothelial Cells, Endothelium, Vascular, Glutathione, Humans, I-kappa B Kinase, Intercellular Adhesion Molecule-1, Organophosphorus Compounds, Oxidative Stress, Reactive Oxygen Species, Tumor Necrosis Factor-alpha, Ubiquinone, Up-Regulation
Check for Full Text / PubMed Unique Identifier (PMID): 17237434
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