Genome-wide Analysis of Foxp3 Target Genes in Developing and Mature Regulatory T Cells.
From: Department of Immunology, University of Washington, Seattle, Washington 98195, USA.
Nature
- Publish Date: Feb 2007
- ISSN: 1476-4687
- Volume: 445
- Issue: 7130
- Pages: 936-40
- Medium: Internet
- Language: English
- Citation (JAMA): Zheng Ye, Josefowicz Steven Z, Kas Arnold, et al. Genome-wide Analysis of Foxp3 Target Genes in Developing and Mature Regulatory T Cells.. Nature Feb 2007;445:936-40
Abstract
Transcription factor Foxp3 (forkhead box P3), restricted in its expression to a specialized regulatory CD4+ T-cell subset (T(R)) with a dedicated suppressor function, controls T(R) lineage development. In humans and mice, Foxp3 deficiency results in a paucity of T(R) cells and a fatal breach in immunological tolerance, causing highly aggressive multi-organ autoimmune pathology. Here, through genome-wide analysis combining chromatin immunoprecipitation with mouse genome tiling array profiling, we identify Foxp3 binding regions for approximately 700 genes and for an intergenically encoded microRNA. We find that a large number of Foxp3-bound genes are up- or downregulated in Foxp3+ T cells, suggesting that Foxp3 acts as both a transcriptional activator and repressor. Foxp3-mediated regulation unique to the thymus affects, among others, genes encoding nuclear factors that control gene expression and chromatin remodelling. In contrast, Foxp3 target genes shared by the thymic and peripheral T(R) cells encode primarily plasma membrane proteins, as well as cell signalling proteins. Together, our studies suggest that distinct transcriptional sub-programmes implemented by Foxp3 establish T(R) lineage during differentiation and its proliferative and functional competence in the periphery.
Mesh Headings (Keywords): Animals, Cell Differentiation, Forkhead Transcription Factors, Gene Expression Profiling, Gene Expression Regulation, Genome, Genomics, Mice, Mice, Inbred C57BL, Oligonucleotide Array Sequence Analysis, Protein Binding, T-Lymphocytes, Regulatory, Thymus Gland
Check for Full Text / PubMed Unique Identifier (PMID): 17237761
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