Localization of Lipids in the Aortic Wall with Imaging Tof-sims.
From: Department of Anatomy and Cell Biology, Göteborg University, P.O. Box 420, SE - 405 30 Göteborg, Sweden.
Biochimica et biophysica acta
- Publish Date: Feb 2007
- ISSN: 0006-3002
- Volume: 1771
- Issue: 2
- Pages: 185-95
- Medium: Print
- Language: English
- Citation (JAMA): Malmberg Per, Börner Katrin, Chen Yun, et al. Localization of Lipids in the Aortic Wall with Imaging Tof-sims.. Biochim. Biophys. Acta Feb 2007;1771:185-95
Abstract
Time-of-flight secondary-ion-mass-spectrometry (TOF-SIMS) was utilized to address the issue of localization of lipids and inorganic ions in healthy rat aorta and human atherosclerotic plaque. Pieces of rat aorta were high pressure frozen, freeze-fractured and freeze dried. The samples were analyzed by imaging TOF-SIMS equipped with a Bi(1-7)(+)-source. Reference lipid samples were analyzed and compared to data obtained by analysis of the rat aorta samples. Fatty acids, cholesterol, oxysterol and diacylglycerols were detected and localized. A heterogeneous lipid distribution could be shown in the aorta, where the lamellae of the aorta, distinguished by imaging of CN(-), appeared enriched in cholesterol, oxysterol and diacylglycerols, while the smooth muscle tissue, identified by imaging of PO(3), appeared enriched in phosphocholine. Palmitic/palmitoleic acid and stearic/oleic acid appeared to be heterogeneously distributed over the aorta with high concentration areas located especially in the tunica media region of the aorta. Human atherosclerotic plaque showed an irregular cholesterol distribution mainly located in spots in the intima region with elongated diacylglycerol regions located mainly in the media region.
Mesh Headings (Keywords): Animals, Aorta, Atherosclerosis, Cholesterol, Fatty Acids, Monounsaturated, Freeze Drying, Humans, Lipids, Oleic Acid, Palmitic Acid, Rats, Rats, Sprague-Dawley, Spectrometry, Mass, Secondary Ion, Stearic Acids
Check for Full Text / PubMed Unique Identifier (PMID): 17240191
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.