Cardiac Side Population Cells Have a Potential to Migrate and Differentiate into Cardiomyocytes in Vitro and in Vivo.
From: Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine, Chuo-ku, Chiba-shi, Chiba 260-8670, Japan.
The Journal of cell biology
- Publish Date: Jan 2007
- ISSN: 0021-9525
- Volume: 176
- Issue: 3
- Pages: 329-41
- Medium: Print
- Language: English
- Citation (JAMA): Oyama Tomomi, Nagai Toshio, Wada Hiroshi, et al. Cardiac Side Population Cells Have a Potential to Migrate and Differentiate into Cardiomyocytes in Vitro and in Vivo.. J. Cell Biol. Jan 2007;176:329-41
Abstract
Side population (SP) cells, which can be identified by their ability to exclude Hoechst 33342 dye, are one of the candidates for somatic stem cells. Although bone marrow SP cells are known to be long-term repopulating hematopoietic stem cells, there is little information about the characteristics of cardiac SP cells (CSPs). When cultured CSPs from neonatal rat hearts were treated with oxytocin or trichostatin A, some CSPs expressed cardiac-specific genes and proteins and showed spontaneous beating. When green fluorescent protein-positive CSPs were intravenously infused into adult rats, many more ( approximately 12-fold) CSPs were migrated and homed in injured heart than in normal heart. CSPs in injured heart differentiated into cardiomyocytes, endothelial cells, or smooth muscle cells (4.4%, 6.7%, and 29% of total CSP-derived cells, respectively). These results suggest that CSPs are intrinsic cardiac stem cells and involved in the regeneration of diseased hearts.
Mesh Headings (Keywords): Adipocytes, Animals, Animals, Newborn, Cell Differentiation, Cell Movement, Cells, Cultured, Endothelial Cells, Gene Expression, Green Fluorescent Proteins, Hydroxamic Acids, Mice, Mice, Inbred C57BL, Myocardium, Myocytes, Cardiac, Myocytes, Smooth Muscle, Osteocytes, Oxytocin, Protein Synthesis Inhibitors, Rats, Rats, Wistar, Stem Cells
Check for Full Text / PubMed Unique Identifier (PMID): 17261849
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