Evidence of Proviral Clearance Following Postpartum Transmission of an Ovine Lentivirus.
From: Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, WA 99164-6630, USA. lherrman@vetmed.wsu.edu
Virology
- Publish Date: May 2007
- ISSN: 0042-6822
- Volume: 362
- Issue: 1
- Pages: 226-34
- Medium: Print
- Language: English
- Citation (JAMA): Herrmann-Hoesing Lynn M, Palmer Guy H, Knowles Donald P, et al. Evidence of Proviral Clearance Following Postpartum Transmission of an Ovine Lentivirus.. Virology May 2007;362:226-34
Abstract
Lentiviral transmission by transfer of infected colostrum and/or milk is considered to be highly efficient. In this study, postpartum transmission of ovine progressive pneumonia virus (OPPV) from 10 naturally infected ewes to their 23 lambs was followed from the perinatal period throughout a four-year period. The lambs were allowed to suckle from their dam from birth through 32 weeks of age. Virus was tracked by virus isolation, quantitative PCR (qPCR), and anti-OPPV antibody responses as measured by cELISA. Cell-associated OPPV was isolated from colostrum/milk cells in 7 out of 10 ewes and provirus envelope (env) loads ranged 8 to 10(5) copies/mug DNA in colostrum/milk cells from the 10 ewes using qPCR. Provirus env loads were also detected in the peripheral circulation of 21 lambs at 8 weeks and two lambs at 22 weeks. The qPCR product at 8 weeks was confirmed as the transmembrane (tm) gene of OPPV by cloning and sequencing. Both cELISA titers ranging from 325 to 3125 and cross-neutralizing antibody titers ranging from 6 to 162 to seven different OPPV strains were found in the colostrum of the 10 ewes. Furthermore, cELISA titers in serum from lambs remained detectable through 32 weeks following the clearance of provirus at 24 weeks. After 32 weeks, both provirus and anti-OPPV antibody responses have subsequently remained undetectable through 4 years of age. These data suggest the clearance of cell-associated lentiviruses from lamb circulation after passive transfer of antibody via colostrum.
Mesh Headings (Keywords): Animals, Antibodies, Viral, Colostrum, DNA, Viral, Disease Transmission, Horizontal, Disease Transmission, Vertical, Enzyme-Linked Immunosorbent Assay, Female, Lentivirus Infections, Lentiviruses, Ovine-Caprine, Milk, Polymerase Chain Reaction, Postpartum Period, Proviruses, RNA, Viral, Sequence Analysis, DNA, Sheep, Sheep Diseases
Check for Full Text / PubMed Unique Identifier (PMID): 17267002
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.