Human Papillomavirus-like Particles Mediate Functional Delivery of Plasmid Dna to Antigen Presenting Cells in Vivo.
From: Department of Medicine, University of Rochester School of Medicine and Dentistry, NY 14642, USA.
Vaccine
- Publish Date: Apr 2007
- ISSN: 0264-410X
- Volume: 25
- Issue: 17
- Pages: 3270-6
- Medium: Print
- Language: English
- Citation (JAMA): Malboeuf Christine M, Simon David A L, Lee Young-Eun Ellen, et al. Human Papillomavirus-like Particles Mediate Functional Delivery of Plasmid Dna to Antigen Presenting Cells in Vivo.. Vaccine Apr 2007;25:3270-6
Abstract
Because recombinant empty viral capsids are potentially attractive vectors for gene therapy, here we examined the ability of human papillomavirus (HPV) virus-like particles (VLPs) to mediate delivery and expression of DNA plasmids in vitro and in vivo. VLP-mediated delivery and expression of a GFP reporter construct in vitro was found to be highly dependent upon the presence of full-length L2 protein within the VLPs. Similarly, expression of GFP and luciferase reporter plasmids in vivo was strongly enhanced by co-administration of L1/L2 VLPs. Interestingly, in these experiments we routinely observed GFP expression in migrating antigen presenting cells (APC) recovered from mice inoculated with GFP plasmid in combination with VLPs, but not in APC recovered from mice inoculated with the plasmid alone. Additional evidence to support this concept was generated in experiments in which co-administration of VLPs with a plasmid designed to express HPV16 E6 oncoprotein was associated with significant enhancement of plasmid-encoded E6-specific cellular immune responses. These findings have implications for the design of vaccines for combined prophylaxis and therapy of HPV-associated diseases, and for other vaccines that rely on the administration of DNA-based immunogens, adjuvants, and/or other factors.
Mesh Headings (Keywords): Animals, Antigen-Presenting Cells, Capsid Proteins, Female, Gene Therapy, Gene Transfer Techniques, Green Fluorescent Proteins, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Oncogene Proteins, Viral, Papillomaviridae, Plasmids, Repressor Proteins, Virion
Check for Full Text / PubMed Unique Identifier (PMID): 17293010
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.