Neurogenin 3 Recruits Cbp Co-activator to Facilitate Histone H3/H4 Acetylation in the Target Gene Insm1.
From: The Research Institute for Children, Children’s Hospital in New Orleans, 200 Henry Clay Avenue, Research and Education Building, Rm. 2211, New Orleans, LA 70118, USA.
FEBS letters
- Publish Date: Mar 2007
- ISSN: 0014-5793
- Volume: 581
- Issue: 5
- Pages: 949-54
- Medium: Print
- Language: English
- Citation (JAMA): Breslin Mary B, Wang Hong-Wei, Pierce Amy, et al. Neurogenin 3 Recruits Cbp Co-activator to Facilitate Histone H3/H4 Acetylation in the Target Gene Insm1.. FEBS Lett. Mar 2007;581:949-54
Abstract
INSM1 is a downstream target gene of neurogenin 3 (ngn3). A promoter construct containing the -426/+40bp region transiently co-transfected into NIH-3T3 cells with a ngn3 expression plasmid resulted in a 12-fold increase in promoter activity. The ngn3/E47 heterodimer selectively binds and activates the E-box3 of the INSM1 promoter. The endogenous ngn3 and CREB-binding protein (CBP) co-activator occupy the INSM1 promoter, resulting in hyper-acetylation of histone H3/H4 chromatin in a human neuroblastoma cell line, IMR-32. Additionally, adenoviral ngn3 can induce endogenous INSM-1 expression in pancreatic ductal carcinoma-1 cells through the recruitment of CBP to the INSM1 promoter and increase the acetylation of the INSM1 promoter region.
Mesh Headings (Keywords): Acetylation, Animals, Base Sequence, Basic Helix-Loop-Helix Transcription Factors, Carrier Proteins, Cell Line, DNA Primers, DNA-Binding Proteins, Gene Expression Regulation, Histones, Humans, Mice, NIH 3T3 Cells, Nerve Tissue Proteins, Promoter Regions (Genetics), Recombinant Proteins, Repressor Proteins, Transcription Factors, Transfection
Check for Full Text / PubMed Unique Identifier (PMID): 17300785
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