Characterization of Leishmania Isolates from Nepalese Patients with Visceral Leishmaniasis.
From: Department of Protozoology, Institute of Tropical Medicine, Nagasaki University, Sakamoto 1-12-4, 852-8523 Nagasaki, Japan. pandey_kishor@hotmail.com
Parasitology research
- Publish Date: May 2007
- ISSN: 0932-0113
- Volume: 100
- Issue: 6
- Pages: 1361-9
- Medium: Print
- Language: English
- Citation (JAMA): Pandey Kishor, Yanagi Testuo, Pandey Basu Dev, et al. Characterization of Leishmania Isolates from Nepalese Patients with Visceral Leishmaniasis.. Parasitol. Res. May 2007;100:1361-9
Abstract
In Nepal, visceral leishmaniasis (VL) is endemic in 13 districts of the central and eastern regions. A total of 166 bone-marrow aspirates were obtained from patients with suspected VL. Ninety-seven were identified as positive by microscopy, and 29 of those were successfully isolated and cultured. We characterized these isolates by molecular analysis and by their ability to infect mice. PCR-restriction fragment length polymorphism analysis of the mini-exon and the cysteine proteinase b gene showed that all isolates were Leishmania donovani, and the restriction pattern of the Nepalese isolates corresponded to the standard Indian strain of L. donovani but differed from that of the Kenyan strain. The single-strand conformation polymorphism analysis of ribosomal internal transcribed spacer showed no genetic heterogeneity within Nepalese isolates. Intraperitoneal inoculation with the promastigotes of all isolates resulted in amastigote proliferation in the spleen of 20 nude mice, of which ten isolates were highly infective, and ten were moderately infective, including one BALB/c mouse. Of the 20 amastigotes isolated from the spleen of nude mice, only the ten highly infective isolates infected BALB/c mice, of which, two isolates were considered to have low infectivity, three isolates were considered to be moderately infective, and five isolates were considered to be highly infective.
Mesh Headings (Keywords): Adult, Aged, Animals, Child, Child, Preschool, Cricetinae, DNA, Protozoan, DNA, Ribosomal Spacer, Female, Humans, Leishmania, Leishmaniasis, Visceral, Male, Mesocricetus, Mice, Mice, Inbred BALB C, Mice, Nude, Middle Aged, Nepal
Check for Full Text / PubMed Unique Identifier (PMID): 17310397
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