Lineage Tracing Using Matrilin-1 Gene Expression Reveals That Articular Chondrocytes Exist As the Joint Interzone Forms.
From: Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, The University of Manchester, Manchester M13 9PT, UK.
Developmental biology
- Publish Date: Apr 2007
- ISSN: 0012-1606
- Volume: 304
- Issue: 2
- Pages: 825-33
- Medium: Print
- Language: English
- Citation (JAMA): Hyde Gareth, Dover Sharon, Aszodi Attila, et al. Lineage Tracing Using Matrilin-1 Gene Expression Reveals That Articular Chondrocytes Exist As the Joint Interzone Forms.. Dev. Biol. Apr 2007;304:825-33
Abstract
We have developed a mouse in which the Cre recombinase gene has been targeted to exon 1 of the matrilin-1 gene (Matn1) to investigate the origins of articular chondrocytes and the development of the knee joint. Analysis of joints from offspring of Matn1-Cre/R26R crosses demonstrated that articular chondrocytes are derived from cells that have never expressed matrilin-1 whereas the remainder of the chondrocytes in the cartilage anlagen expresses matrilin-1. A band of chondrocytes adjacent to the developing interzone in the E13.5 day knee joint became apparent because these chondrocytes did not turn on expression of matrilin-1 in contrast to the other chondrocytes of the anlagen. The chondrocytes of the presumptive articular surface therefore appear to arise directly from a subpopulation of early chondrocytes that do not activate matrilin-1 expression rather than by redifferentiation from the flattened cells of the interzone. In addition, lineage tracing using both Matn1-Cre/R26R and Col2a1-Cre/R26R lines indicated that non-cartilaginous structures in the knee such as cruciate ligament, synovium and some blood vessels are formed by cells derived from the early chondrocytes of the anlagen.
Mesh Headings (Keywords): Animals, Animals, Newborn, Cartilage, Articular, Cell Differentiation, Cell Lineage, Chondrocytes, Exons, Extracellular Matrix Proteins, Glycoproteins, Joints, Mice, Mice, Transgenic, Synovial Membrane
Check for Full Text / PubMed Unique Identifier (PMID): 17313942
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