The Bacillus Subtilis Natk-natr Two-component System Regulates Expression of the Natab Operon Encoding an Abc Transporter for Sodium Ion Extrusion.
From: Institute of Oceanic Research and Development, Tokai University, 3-20-1 Orido-Shimizu, Shizuoka 424-8610, Japan. oguram@scc.u-tokai.ac.jp
Microbiology (Reading, England)
- Publish Date: Mar 2007
- ISSN: 1350-0872
- Volume: 153
- Issue: Pt 3
- Pages: 667-75
- Medium: Print
- Language: English
- Citation (JAMA): Ogura Mitsuo, Tsukahara Kensuke, Hayashi Kentaro, et al. The Bacillus Subtilis Natk-natr Two-component System Regulates Expression of the Natab Operon Encoding an Abc Transporter for Sodium Ion Extrusion.. Microbiology (Reading, Engl.) Mar 2007;153:667-75
Abstract
A previous microarray analysis suggested that multicopy yccH, encoding a function-unknown response regulator, enhances expression of natAB, which encodes a two-gene ATP-binding cassette transporter involved in the extrusion of sodium ions. The two-component regulatory system YccG-YccH was therefore renamed NatK-NatR. Here, this observation was confirmed by a lacZ fusion analysis using a strain carrying natA-lacZ. Further, in both natK and natR mutants, natA-lacZ expression was completely abolished, indicating that the NatK-NatR system positively regulates the expression of natAB. In a gel retardation analysis, NatR bound to the natA promoter region. Using purified His-tagged NatR, DNase I footprinting analysis of the natA promoter region suggested that a direct repeat of [TTCA(G)CGACA], separated by a 12 bp space, would be recognized by NatR. Deleted and mutagenized promoter regions of natA were analysed using a lacZ fusion, and it was confirmed that the direct repeat is critical for natA activation by NatR.
Mesh Headings (Keywords): ATP-Binding Cassette Transporters, Adaptation, Physiological, Artificial Gene Fusion, Bacillus subtilis, Bacterial Proteins, Binding Sites, DNA Footprinting, DNA, Bacterial, DNA-Binding Proteins, Gene Expression Regulation, Bacterial, Mutation, Oligonucleotide Array Sequence Analysis, Operon, Promoter Regions (Genetics), Protein Binding, Sequence Deletion, Sodium, Transcription Factors, beta-Galactosidase
Check for Full Text / PubMed Unique Identifier (PMID): 17322186
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