The Interaction Between Stargazin and Psd-95 Regulates Ampa Receptor Surface Trafficking.
From: Physiologie Cellulaire de la Synapse, UMR 5091 CNRS - Institut François Magendie, Université Bordeaux, Bordeaux 33077, France.
Neuron
- Publish Date: Mar 2007
- ISSN: 0896-6273
- Volume: 53
- Issue: 5
- Pages: 719-34
- Medium: Print
- Language: English
- Citation (JAMA): Bats Cecile, Groc Laurent, Choquet Daniel, et al. The Interaction Between Stargazin and Psd-95 Regulates Ampa Receptor Surface Trafficking.. Neuron Mar 2007;53:719-34
Abstract
Accumulation of AMPA receptors at synapses is a fundamental feature of glutamatergic synaptic transmission. Stargazin, a member of the TARP family, is an AMPAR auxiliary subunit allowing interaction of the receptor with scaffold proteins of the postsynaptic density, such as PSD-95. How PSD-95 and Stargazin regulate AMPAR number in synaptic membranes remains elusive. We show, using single quantum dot and FRAP imaging in live hippocampal neurons, that exchange of AMPAR by lateral diffusion between extrasynaptic and synaptic sites mostly depends on the interaction of Stargazin with PSD-95 and not upon the GluR2 AMPAR subunit C terminus. Disruption of interactions between Stargazin and PSD-95 strongly increases AMPAR surface diffusion, preventing AMPAR accumulation at postsynaptic sites. Furthermore, AMPARs and Stargazin diffuse as complexes in and out synapses. These results propose a model in which the Stargazin-PSD-95 interaction plays a key role to trap and transiently stabilize diffusing AMPARs in the postsynaptic density.
Mesh Headings (Keywords): Animals, Calcium Channels, Cells, Cultured, Excitatory Postsynaptic Potentials, Fluorescence Recovery After Photobleaching, Gene Expression, Green Fluorescent Proteins, Hippocampus, Intracellular Signaling Peptides and Proteins, Membrane Proteins, Mice, Neurons, Protein Binding, Protein Transport, Receptor Aggregation, Receptors, AMPA, Recombinant Proteins, Synapses
Check for Full Text / PubMed Unique Identifier (PMID): 17329211
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