Identification of an Immunodominant Cd4+ T Cell Epitope in the Vp6 Protein of Rotavirus Following Intranasal Immunization of Balb/C Mice.
From: Division of Infectious Diseases, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH 45229, USA.
Virology
- Publish Date: Jul 2007
- ISSN: 0042-6822
- Volume: 363
- Issue: 2
- Pages: 410-8
- Medium: Print
- Language: English
- Citation (JAMA): McNeal Monica M, Basu Mitali, Bean Judy A, et al. Identification of an Immunodominant Cd4+ T Cell Epitope in the Vp6 Protein of Rotavirus Following Intranasal Immunization of Balb/C Mice.. Virology Jul 2007;363:410-8
Abstract
The only lymphocytes required for protection against fecal rotavirus shedding after intranasal immunization of BALB/c (H-2(d)) mice with a chimeric rotavirus VP6 protein (MBPColon, two colonsVP6) and the mucosal adjuvant LT(R192G) are CD4(+) T cells. The purpose of this study was to identify CD4(+) T cell epitopes within VP6 that might be responsible for this protection. To make this determination, spleen cells obtained from BALB/c mice following intranasal immunization with MBPColon, two colonsVP6/LT(R192G) were stimulated in vitro with either MBPColon, two colonsVP6 or overlapping VP6 peptides containing
Mesh Headings (Keywords): Administration, Intranasal, Amino Acid Sequence, Animals, Antigens, Viral, CD4-Positive T-Lymphocytes, Capsid Proteins, Cells, Cultured, Cytokines, Epitopes, T-Lymphocyte, Female, Immunity, Cellular, Immunization Schedule, Immunodominant Epitopes, Lymphocyte Count, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Peptides, Rotavirus, Rotavirus Infections, Rotavirus Vaccines, Spleen, Vaccination, Vaccines, Synthetic
Check for Full Text / PubMed Unique Identifier (PMID): 17337285
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