Antigiardial Activity of Ocimum Basilicum Essential Oil.
From: Instituto de Microbiologia Prof. Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 219491-590, Brazil.
Parasitology research
- Publish Date: Jul 2007
- ISSN: 0932-0113
- Volume: 101
- Issue: 2
- Pages: 443-52
- Medium: Print
- Language: English
- Citation (JAMA): de Almeida Igor, Alviano Daniela Sales, Vieira Danielle Pereira, et al. Antigiardial Activity of Ocimum Basilicum Essential Oil.. Parasitol. Res. Jul 2007;101:443-52
Abstract
In this study, we investigated the effects of Ocimum basilicum essential oil on Giardia lamblia and on the modulation of the interaction of these parasites by peritoneal mouse macrophage. The essential oil (2 mg/ml) and its purified substances demonstrated antigiardial activity. Linalool (300 microg/ml), however, was able to kill 100% parasites after 1 h of incubation, which demonstrates its high antigiardial potential. Pretreatment of peritoneal mouse macrophages with 2 mg/ml essential oil dilution reduced in 79% the association index between these macrophages and G. lamblia, with a concomitant increase by 153% on nitric oxide production by the G. lamblia-ingested macrophages. The protein profiles and proteolitic activity of these parasite trophozoites, previously treated or not with 2 mg/ml essential oil or with the purified fractions, were also determined. After 1 and 2 h of incubation, proteins of lysates and culture supernatants revealed significant differences in bands patterns when compared to controls. Besides, the proteolitic activity, mainly of cysteine proteases, was clearly inhibited by the essential oil (2 mg/ml) and the purified linalool (300 microg/ml). These results suggest that, with G. lamblia, the essential oil from O. basilicum and its purified compounds, specially linalool, have a potent antimicrobial activity.
Mesh Headings (Keywords): Animals, Antiprotozoal Agents, Cell Survival, Cells, Cultured, Female, Giardia lamblia, Macrophages, Peritoneal, Mice, Monoterpenes, Nitric Oxide, Ocimum basilicum, Oils, Volatile, Plant Extracts, Protozoan Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 17342533
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.