Enhanced Proliferation of Astrocytes from Beta(2)-adrenergic Receptor Knockout Mice is Influenced by the Igf System.
From: Department of Neurology, University Medical Center Groningen, Groningen, the Netherlands. d.chesik@med.umcg.nl
Journal of neurochemistry
- Publish Date: Mar 2007
- ISSN: 0022-3042
- Volume: 100
- Issue: 6
- Pages: 1555-64
- Medium: Print
- Language: English
- Citation (JAMA): Chesik Daniel, Glazenburg Lisa, De Keyser Jacques, et al. Enhanced Proliferation of Astrocytes from Beta(2)-adrenergic Receptor Knockout Mice is Influenced by the Igf System.. J. Neurochem. Mar 2007;100:1555-64
Abstract
In the present study, we investigated the IGF system in neonatal astrocytes derived from mice with a targeted disruption of the beta-2 adrenergic receptor (beta(2)AR). beta(2)AR knockout astrocytes demonstrated higher proliferation rates and increased expression of the astrogliotic marker GFAP, as compared with wild-type cells. beta(2)AR deletion also regulated molecules of the IGF system. Although IGF-1 levels remained unaltered, IGF-2 and type 1 IGF receptor expression was increased in beta(2)AR knockout cells. Furthermore, conditioned medium from knockout astrocytes contained lower levels of IGF binding protein-2 and -4. Our data suggest a deficit of beta(2)AR on astrocytes, as previously reported in multiple sclerosis, may have implications on proliferative status of astrocytes, a feature that might be attributed to regulation of IGF mitogenic actions.
Mesh Headings (Keywords): Animals, Animals, Newborn, Astrocytes, Cell Count, Cell Proliferation, Cells, Cultured, Cerebral Cortex, Culture Media, Conditioned, Dose-Response Relationship, Drug, Gene Expression Regulation, Insulin-Like Growth Factor Binding Protein 1, Insulin-Like Growth Factor Binding Protein 4, Mice, Mice, Knockout, RNA, Messenger, Receptor, IGF Type 1, Receptor, IGF Type 2, Receptors, Adrenergic, beta-2, Reverse Transcriptase Polymerase Chain Reaction
Check for Full Text / PubMed Unique Identifier (PMID): 17348863
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.