Applications of Fluorescence Microscopy to Single Bacterial Cells.
From: Department of Microbiology, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, Room 1218, New York, NY 10032, USA.
Research in microbiology
- Publish Date: Apr 2007
- ISSN: 0923-2508
- Volume: 158
- Issue: 3
- Pages: 187-94
- Medium: Print
- Language: English
- Citation (JAMA): Meyer Pablo, Dworkin Jonathan, et al. Applications of Fluorescence Microscopy to Single Bacterial Cells.. Res. Microbiol. Apr 2007;158:187-94
Abstract
Fluorescence-imaging techniques are now being applied to single bacterial cells for the determination of in vivo kinase and transcription activity, protein-protein interactions, protein localization and protein mobility. Time-lapse imaging is being used at a population level to study heterogeneities, colony morphologies and the genetic networks underlying these phenomena. We will discuss here the possibilities, advantages and limitations of such techniques, focusing on the use of green fluorescent protein and its spectral variants.
Mesh Headings (Keywords): Bacteria, Bacterial Proteins, Fluorescence Resonance Energy Transfer, Luminescent Proteins, Microscopy, Fluorescence, Recombinant Fusion Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 17349779
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