Synergism Between Hydrogen Sulfide (H(2)s) and Nitric Oxide (No) in Vasorelaxation Induced by Stonustoxin (Sntx), a Lethal and Hypotensive Protein Factor Isolated from Stonefish Synanceja Horrida Venom.
From: Department of Biochemistry, National University of Singapore, Blk MD4A, 10 Kent Ridge Crescent, Singapore.
Life sciences
- Publish Date: Apr 2007
- ISSN: 0024-3205
- Volume: 80
- Issue: 18
- Pages: 1664-8
- Medium: Print
- Language: English
- Citation (JAMA): Liew H C, Khoo H E, Moore P K, et al. Synergism Between Hydrogen Sulfide (H(2)s) and Nitric Oxide (No) in Vasorelaxation Induced by Stonustoxin (Sntx), a Lethal and Hypotensive Protein Factor Isolated from Stonefish Synanceja Horrida Venom.. Life Sci. Apr 2007;80:1664-8
Abstract
Stonustoxin (SNTX) is a 148 kDa, dimeric, hypotensive and lethal protein factor isolated from the venom of the stonefish Synanceja horrida. SNTX (10-320 ng/ml) progressively causes relaxation of endothelium-intact, phenylephrine (PE)-precontracted rat thoracic aortic rings. The SNTX-induced vasorelaxation was inhibited by L-N(G)-nitro arginine methyl ester (L-NAME), suggesting that nitric oxide (NO) contributes to the SNTX-induced response. Interestingly, D, L-proparglyglycine (PAG) and beta-cyano-L-alanine (BCA), irreversible and competitive inhibitors of cystathionine-gamma-lyase (CSE) respectively, also inhibited SNTX-induced vasorelaxation, indicating that H(2)S may also play a part in the effect of SNTX. The combined use of L-NAME with PAG or BCA showed that H(2)S and NO act synergistically in effecting SNTX-induced vasorelaxation.
Mesh Headings (Keywords): Animals, Aorta, Thoracic, Cystathionine gamma-Lyase, Enzyme Inhibitors, Fish Venoms, Fishes, Poisonous, Hydrogen Sulfide, Male, Nitric Oxide, Organ Culture Techniques, Phenylephrine, Rats, Rats, Sprague-Dawley, Vasoconstrictor Agents, Vasodilation
Check for Full Text / PubMed Unique Identifier (PMID): 17350050
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