Characterization of Mouse Neuronal Ca2+/Calmodulin Kinase Ii Inhibitor Alpha.
From: Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
Brain research
- Publish Date: May 2007
- ISSN: 0006-8993
- Volume: 1148
- Issue:
- Pages: 38-42
- Medium: Print
- Language: English
- Citation (JAMA): Saha Sougata, Datta Krishnalekha, Rangarajan Pundi, et al. Characterization of Mouse Neuronal Ca2+/Calmodulin Kinase Ii Inhibitor Alpha.. Brain Res. May 2007;1148:38-42
Abstract
We have overexpressed an 8.5-kDa mouse Ca(2+)/calmodulin kinase II inhibitor alpha protein (mCaMKIINalpha) in Escherichia coli and demonstrate that the recombinant protein is a potent inhibitor of Ca(2+)/calmodulin kinase II (CaMKII) in vitro. However, antibodies raised against recombinant mCaMKIINalpha react with an approximately 37-kDa protein present in mouse brain. The pattern of expression of the approximately 37-kDa protein is similar to that of mCaMKIINalpha mRNA as both are expressed in normal but not Japanese encephalitis virus (JEV)-infected mouse brain. Subcellular localization studies indicate that the approximately 37-kDa protein is present in the post-synaptic density (PSD) where mCaMKIIalpha is known to perform key regulatory functions. We conclude that the approximately 37-kDa protein identified in this study is mCaMKIINalpha and its localization in the PSD indicates a novel role for this protein in the regulation of neuronal CaMKIIalpha.
Mesh Headings (Keywords): Amino Acid Sequence, Animals, Brain, Brain Chemistry, Calcium Signaling, Calcium-Calmodulin-Dependent Protein Kinase Type 2, Calcium-Calmodulin-Dependent Protein Kinases, Carrier Proteins, Encephalitis, Japanese, Mice, Molecular Sequence Data, Neurons, RNA, Messenger, Recombinant Fusion Proteins, Synaptic Membranes, Synaptic Transmission
Check for Full Text / PubMed Unique Identifier (PMID): 17350603
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