The Macrophage Cd163 Surface Glycoprotein is an Erythroblast Adhesion Receptor.
From: Department of Molecular Cell Biology, Vrije Universiteit Medical Center, Amsterdam, the Netherlands.
Blood
- Publish Date: Jun 2007
- ISSN: 0006-4971
- Volume: 109
- Issue: 12
- Pages: 5223-9
- Medium: Print
- Language: English
- Citation (JAMA): Fabriek Babs O, Polfliet Machteld M J, Vloet Rianka P M, et al. The Macrophage Cd163 Surface Glycoprotein is an Erythroblast Adhesion Receptor.. Blood Jun 2007;109:5223-9
Abstract
Erythropoiesis occurs in erythroblastic islands, where developing erythroblasts closely interact with macrophages. The adhesion molecules that govern macrophage-erythroblast contact have only been partially defined. Our previous work has implicated the rat ED2 antigen, which is highly expressed on the surface of macrophages in erythroblastic islands, in erythroblast binding. In particular, the monoclonal antibody ED2 was found to inhibit erythroblast binding to bone marrow macrophages. Here, we identify the ED2 antigen as the rat CD163 surface glycoprotein, a member of the group B scavenger receptor cysteine-rich (SRCR) family that has previously been shown to function as a receptor for hemoglobin-haptoglobin (Hb-Hp) complexes and is believed to contribute to the clearance of free hemoglobin. CD163 transfectants and recombinant protein containing the extracellular domain of CD163 supported the adhesion of erythroblastic cells. Furthermore, we identified a 13-amino acid motif (CD163p2) corresponding to a putative interaction site within the second scavenger receptor domain of CD163 that could mediate erythroblast binding. Finally, CD163p2 promoted erythroid expansion in vitro, suggesting that it enhanced erythroid proliferation and/or survival, but did not affect differentiation. These findings identify CD163 on macrophages as an adhesion receptor for erythroblasts in erythroblastic islands, and suggest a regulatory role for CD163 during erythropoiesis.
Mesh Headings (Keywords): Amino Acid Motifs, Animals, Antigens, CD, Antigens, Differentiation, Myelomonocytic, Binding Sites, Bone Marrow Cells, Cell Adhesion, Cell Proliferation, Erythroblasts, Erythropoiesis, Macrophages, Membrane Glycoproteins, Membrane Proteins, Rats, Receptors, Cell Surface
Check for Full Text / PubMed Unique Identifier (PMID): 17353345
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.