Identification of the Tpo1 Gene in Yeast, and Its Human Orthologue Tetran, Which Cause Resistance to Nsaids.
From: Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 862-0973, Japan.
FEBS letters
- Publish Date: Apr 2007
- ISSN: 0014-5793
- Volume: 581
- Issue: 7
- Pages: 1457-63
- Medium: Print
- Language: English
- Citation (JAMA): Mima Shinji, Ushijima Hironori, Hwang Hyun-Jung, et al. Identification of the Tpo1 Gene in Yeast, and Its Human Orthologue Tetran, Which Cause Resistance to Nsaids.. FEBS Lett. Apr 2007;581:1457-63
Abstract
Non-steroidal anti-inflammatory drugs (NSAIDs), such as indomethacin, have serious gastrointestinal side effects. Since their direct cytotoxicity was suggested to be involved in this side effect, we here tried to identify NSAID-resistant genes. We screened for Saccharomyces cerevisiae genes whose overexpression causes indomethacin resistance and identified the TPO1 gene, which encodes a major facilitator superfamily transporter. Its overexpression or deletion made yeast cells resistant or sensitive, respectively, to some NSAIDs. A BLAST search identified the possible human orthologue of Tpo1p, tetracycline transporter-like protein (TETRAN), whose overexpression in cultured human cells caused resistance to some NSAIDs, suggesting that TETRAN is an efflux pump for some NSAIDs.
Mesh Headings (Keywords): Amino Acid Sequence, Anti-Inflammatory Agents, Non-Steroidal, Cells, Cultured, Computational Biology, Drug Resistance, Gene Deletion, Genetic Screening, Humans, Membrane Transport Proteins, Molecular Sequence Data, Mutation, RNA, Messenger, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 17362938
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