Different Kinetics of Blimp-1 Induction in B Cell Subsets Revealed by Reporter Gene.
From: Immunology Division, Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria, Australia.
Journal of immunology (Baltimore, Md. : 1950)
- Publish Date: Apr 2007
- ISSN: 0022-1767
- Volume: 178
- Issue: 7
- Pages: 4104-11
- Medium: Print
- Language: English
- Citation (JAMA): Fairfax Kirsten A, Corcoran Lynn M, Pridans Clare, et al. Different Kinetics of Blimp-1 Induction in B Cell Subsets Revealed by Reporter Gene.. J. Immunol. Apr 2007;178:4104-11
Abstract
The transcriptional repressor Blimp-1 (B lymphocyte-induced maturation protein 1) has been described as a “master regulator” of B cell differentiation into Ab-secreting cells (ASCs). Although there is mounting evidence for the importance and necessity of Blimp-1 in plasma cell development, there is uncertainty as to the role it plays in B cell differentiation of B cell subsets and the way in which it may interact with other transcription factors such as Pax5 and Bcl6 during ASC differentiation. Using a mouse expressing GFP under the control of the Blimp-1 regulatory elements (Blimp-1(GFP/+)), we examined the kinetics of Blimp-1 up-regulation in purified B cell subsets following activation. B1 cells showed the most rapid and pronounced up-regulation of Blimp-1 in response to the mitogens tested, followed by marginal zone B cells and then conventional B2 cells. Interestingly, only B1 cells substantially up-regulated Blimp-1 expression in response to CpG. B1 cells secreted negligible Ig upon isolation but were able to up-regulate Blimp-1 and initiate Ig secretion within 28 h of stimulation. Also of interest, B1 cells have a transcriptional factor profile that is intermediate between a naive B cell and an ASC, indicative of the semiactivated state of B1 cells. Transferred naive Blimp-1(GFP/+) B1 and B2 cells both gave rise to ASCs in the bone marrow, suggesting no intrinsic barriers to B1 cell entry into the long-lived ASC compartment.
Mesh Headings (Keywords): Animals, B-Cell-Specific Activator Protein, B-Lymphocyte Subsets, Bone Marrow, Cell Differentiation, DNA-Binding Proteins, Genes, Reporter, Green Fluorescent Proteins, Immunoglobulins, Kinetics, Lymphocyte Activation, Mice, Mice, Mutant Strains, Regulatory Elements, Transcriptional, Repressor Proteins, Transcription Factors, Up-Regulation
Check for Full Text / PubMed Unique Identifier (PMID): 17371965
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