Identification of Phostensin, a Pp1 F-actin Cytoskeleton Targeting Subunit.
From: Department of Life Science, National Chung Hsing University, Taichung, Taiwan.
Biochemical and biophysical research communications
- Publish Date: May 2007
- ISSN: 0006-291X
- Volume: 356
- Issue: 3
- Pages: 594-8
- Medium: Print
- Language: English
- Citation (JAMA): Kao Shu-Chen, Chen Chun-Yu, Wang Shiu-Lan, et al. Identification of Phostensin, a Pp1 F-actin Cytoskeleton Targeting Subunit.. Biochem. Biophys. Res. Commun. May 2007;356:594-8
Abstract
We have identified a novel protein, protein phosphatase 1 F-actin cytoskeleton targeting subunit (phostensin). This protein is encoded by KIAA1949 and was found to associate with protein phosphatase 1 (PP1) in the yeast two-hybrid assay, co-immunoprecipitation, and GST pull-down assay. Northern blot analysis revealed that phostensin mRNA was predominantly distributed in leukocytes and spleen, and phostensin protein was present in crude extracts of human peripheral leukocytes. Immunofluorescence microscopic analysis revealed that the phostensin/PP1 complex was conspicuously localized with the actin cytoskeleton at the cell periphery in Madin-Darby canine kidney (MDCK) epithelial cells. Taken together, our data shows that phostensin targets PP1 to F-actin cytoskeleton. The phostensin/PP1 complex may play a vital role in modulation of actin rearrangements.
Mesh Headings (Keywords): Actins, Amino Acid Sequence, Animals, Cells, Cultured, Dogs, Fluorescent Antibody Technique, Indirect, Humans, Leukocytes, Phosphoprotein Phosphatases, Protein Phosphatase 1, Two-Hybrid System Techniques
Check for Full Text / PubMed Unique Identifier (PMID): 17374523
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