Determination of Biogenic Amines in Squid and White Prawn by High-performance Liquid Chromatography with Postcolumn Derivatization.
From: Department of Food Science and Technology, Ocean University of China, Qingdao, Shandong Province, 266003, People’s Republic of China.
Journal of agricultural and food chemistry
- Publish Date: Apr 2007
- ISSN: 0021-8561
- Volume: 55
- Issue: 8
- Pages: 3083-8
- Medium: Print
- Language: English
- Citation (JAMA): Zhao Qing-Xi, Xu Jie, Xue Chang-Hu, et al. Determination of Biogenic Amines in Squid and White Prawn by High-performance Liquid Chromatography with Postcolumn Derivatization.. J. Agric. Food Chem. Apr 2007;55:3083-8
Abstract
A simple method was developed for the determination of biogenic amines in aquatic food products using a reverse-phase high-performance liquid chromatography with postcolumn automatic o-phthalaldehyde derivatization and fluorescence detection. The linearity, repeatability, and recovery of the method for seven amines (tyramine, putrescine, cadaverine, histamine, agmatine, spermidine, and spermine) were evaluated. This optimized method was applied to detect biogenic amines in squid and white prawn during refrigerated storage. Sensory analysis, pH value, and total volatile base nitrogen value were combined to evaluate the freshness quality of these two raw materials. Agmatine and cadaverine in squid, cadaverine, and putrescine in white prawn were the most obviously changed amines during the storage at two different temperatures, and these biogenic amines could be the effective quality indicators for the freshness of the raw aquatic materials.
Mesh Headings (Keywords): Animals, Biogenic Amines, Chromatography, High Pressure Liquid, Cold, Crustacea, Decapodiformes, Food Preservation, Reproducibility of Results
Check for Full Text / PubMed Unique Identifier (PMID): 17381105
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