Different Obscurin Isoforms Localize to Distinct Sites at Sarcomeres.
From: Department of Physiology, University of Maryland School of Medicine, 655 W. Baltimore St, MD 21201, USA.
FEBS letters
- Publish Date: Apr 2007
- ISSN: 0014-5793
- Volume: 581
- Issue: 8
- Pages: 1549-54
- Medium: Print
- Language: English
- Citation (JAMA): Bowman Amber L, Kontrogianni-Konstantopoulos Aikaterini, Hirsch Sara S, et al. Different Obscurin Isoforms Localize to Distinct Sites at Sarcomeres.. FEBS Lett. Apr 2007;581:1549-54
Abstract
We used four antibodies to regions of obscurin isoforms A and B, encoded by the obscurin gene, to investigate the location of these proteins in skeletal myofibers at resting and stretched lengths. Obscurin A ( approximately 800 kDa) which was recognized by antibodies generated to the N-terminal, Rho-GEF, and the non-modular C-terminal domain that lacks the kinase-like domains, localizes at the level of the M-band. Obscurin B ( approximately 900 kDa) which has the N-terminal, Rho-GEF, and the C-terminal kinase-like domains, localizes at the level of the A/I junction. Additional isoforms, which lack one or more of these epitopes, are present at the Z-disk and Z/I junction.
Mesh Headings (Keywords): Animals, Muscle Proteins, Muscle, Skeletal, Protein Isoforms, Sarcomeres
Check for Full Text / PubMed Unique Identifier (PMID): 17382936
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