Acute Myocardial Infarction in Mice: Assessment of Transmurality by Strain Rate Imaging.
From: Faculté de Médecine Lyon Nord, INSERM E 0226, 8 Avenue Rockefeller, 69373 Lyon cedex 8, France.
American journal of physiology. Heart and circulatory physiology
- Publish Date: Jul 2007
- ISSN: 0363-6135
- Volume: 293
- Issue: 1
- Pages: H496-502
- Medium: Print
- Language: English
- Citation (JAMA): Thibault Hélène, Gomez Ludovic, Donal Erwan, et al. Acute Myocardial Infarction in Mice: Assessment of Transmurality by Strain Rate Imaging.. Am. J. Physiol. Heart Circ. Physiol. Jul 2007;293:H496-502
Abstract
In vivo evaluation of the transmural extension of myocardial infarction (TEI) is crucial to prediction of viability and prognosis. With the rise of transgenic technology, murine myocardial infarction (MI) models are increasingly used. Our study aimed to evaluate systolic strain rate (SR), a new parameter of regional function, to quantify TEI in a murine model of acute MI induced by various durations of ischemia followed by 24 h of reperfusion. Global and regional left ventricular (LV) function were assessed by echocardiography (13 MHz, Vivid 7, GE) in 4 groups of wild-type mice (C57BL/6, 2 mo old): a sham-treated group (n = 10) and three MI groups [30 (n = 11), 60 (n = 10), and 90 (n = 9) min of left coronary artery occlusion]. Conventional LV dimensions, anterior wall (AW) thickening, and peak systolic SR were measured before and 24 h after reperfusion. Area at risk (AR) was measured by blue dye and infarct size (area of necrosis, AN) and TEI by triphenyltetrazolium chloride staining. AN increased with ischemia duration (25 +/- 2%, 56 +/- 5%, 71 +/- 6% of AR for 30, 60, and 90 min, respectively; P < 0.05). LV end-diastolic volume significantly increased with ischemia duration (30 +/- 5, 34 +/- 5, 43 +/- 5 microl; P < 0.05), whereas LV ejection fraction decreased (63 +/- 5%, 58 +/- 6%, 46 +/- 5%; P < 0.05). AW thickening decrease was not influenced by ischemia duration. Conversely, systolic SR decreased with ischemia duration (13 +/- 5, 4 +/- 3, -2 +/- 6 s(-1); P < 0.05) and was significantly correlated with TEI (r = 0.89, P < 0.01). Receiver operating characteristic (ROC) curves identified systolic SR as the most accurate parameter to predict TEI. In conclusion, in a murine model of MI, SR imaging is superior to conventional echocardiography to predict TEI early after MI.
Mesh Headings (Keywords): Animals, Echocardiography, Elasticity, Image Interpretation, Computer-Assisted, Mice, Mice, Inbred C57BL, Myocardial Infarction, Ventricular Dysfunction, Left
Check for Full Text / PubMed Unique Identifier (PMID): 17384134
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.