Ectopic Neocartilage Formation from Predifferentiated Human Adipose Derived Stem Cells Induced by Adenoviral-mediated Transfer of Htgf Beta2.
From: Orthopaedics Department, Peking University, Third Hospital, Beijing 100083, PR China.
Biomaterials
- Publish Date: Jul 2007
- ISSN: 0142-9612
- Volume: 28
- Issue: 19
- Pages: 2994-3003
- Medium: Print
- Language: English
- Citation (JAMA): Jin Xiao bing, Sun Yong sheng, Zhang Ke, et al. Ectopic Neocartilage Formation from Predifferentiated Human Adipose Derived Stem Cells Induced by Adenoviral-mediated Transfer of Htgf Beta2.. Biomaterials Jul 2007;28:2994-3003
Abstract
Chondrogenic potential of human adipose derived stem cells (hASCs) makes them a possible source of seeding cells for cartilage tissue engineering. In this study, chondrogenic differentiation of hASCs induced by transduction with replication-deficient adenovirus carrying human transforming growth factor beta2 (Ad5-hTGF beta2) was demonstrated by RT-PCR, immunohistochemistry staining, biochemical and western blot analysis. To evaluate if the in vitro differentiated hASCs could keep their chondrocytic phenotype and produce neo-cartilage in vivo, predifferentiated hASCs were seeded in different scaffolds and implanted in subcutaneous pockets on the dorsum of nude mice. After 4 and 12 weeks culture in vivo, specimens were harvested and examined by histological and immunohistochemical analysis, cartilage-like tissue formation was only found in alginate gel and PLGA/alginate compound groups, in PLGA group, fibrous tissues and angiogenesis ingrowth were observed. These findings demonstrated that adenovirus-mediated hTGF beta2 gene transfer could induce hASCs into a chondrogenic lineage in vitro, however, this predifferentiation did not guarantee ectopic cartilage formation in vivo unless appropriate three-dimensional scaffolds were used as the cell carry vehicles.
Mesh Headings (Keywords): Adenoviridae, Adipose Tissue, Aged, Alginates, Animals, Biocompatible Materials, Cartilage, Cell Differentiation, Cells, Cultured, Chondrogenesis, Genetic Vectors, Glucuronic Acid, Hexuronic Acids, Humans, Lactic Acid, Mice, Mice, Inbred BALB C, Mice, Nude, Middle Aged, Polyglycolic Acid, Polymers, Stem Cells, Tissue Engineering, Transforming Growth Factor beta2
Check for Full Text / PubMed Unique Identifier (PMID): 17399784
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