Mannose-binding Lectin A-deficient Mice Have Abrogated Antigen-specific Igm Responses and Increased Susceptibility to a Nematode Infection.
From: Department of Medicine, Imperial College London, St. Mary’s Campus, London, UK.
Journal of immunology (Baltimore, Md. : 1950)
- Publish Date: Apr 2007
- ISSN: 0022-1767
- Volume: 178
- Issue: 8
- Pages: 5116-23
- Medium: Print
- Language: English
- Citation (JAMA): Carter Tim, Sumiya Michiko, Reilly Kerri, et al. Mannose-binding Lectin A-deficient Mice Have Abrogated Antigen-specific Igm Responses and Increased Susceptibility to a Nematode Infection.. J. Immunol. Apr 2007;178:5116-23
Abstract
To investigate the role of mannose-binding lectin-A (MBL-A) in protection against infectious disease, MBL-A(-/-)-deficient mice were generated. Using a well-characterized mouse model of human filarial nematode infection, nematode survival and protective immune responses were tested in vivo. Blood-borne Brugia malayi microfilariae survived for significantly longer time periods in MBL-A(-/-) than in wild-type (WT) mice. However, no differences in either splenic cytokine responses or induction of leukocytes in the blood were observed. A profound abrogation of Ag-specific IgM levels was measured in B. malayi-infected MBL-A(-/-) mice, and some IgG isotypes were higher than those observed in WT animals. To establish whether there was a defect in Ab responses per se in MBL-A(-/-) mice or the effect was specific to filarial infection, we immunized these mice with OVA or a carbohydrate-free protein. Significantly, Ag-specific IgM responses were defective to both of these Ags, and Ag-specific IgG responses were largely unaffected. Furthermore, in naive mice, total IgM levels did not differ between MBL-A(-/-) and WT mice. This article describes the first demonstration that MBL-A may function independently of MBL-C and suggests that MBL-A, like other C-type lectins and members of the complement cascade, is intimately involved in the priming of the humoral Ab response.
Mesh Headings (Keywords): Animals, Antibodies, Helminth, Brugia malayi, Complement C3, Disease Susceptibility, Filariasis, Immunoglobulin Isotypes, Immunoglobulin M, Leukocyte Count, Mannose-Binding Lectin, Mice, Mice, Inbred C57BL
Check for Full Text / PubMed Unique Identifier (PMID): 17404294
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.