Immunocytochemistry on Suprachiasmatic Nucleus Slices.
From: Departrment of Molecular and Integrative Neuroscience, Imperial College London, UK.
Methods in molecular biology (Clifton, N.J.)
- Publish Date: 2007
- ISSN: 1064-3745
- Volume: 362
- Issue:
- Pages: 549-59
- Medium: Print
- Language: English
- Citation (JAMA): Llamosas Marta Muñoz, et al. Immunocytochemistry on Suprachiasmatic Nucleus Slices.. Methods Mol. Biol. 2007;362:549-59
Abstract
Immunocytochemistry (ICC) is a sensitive and powerful method that is used to localize and identify cells containing a particular antigen. This chapter is dedicated to ICC of suprachiasmatic nucleus (SCN) slices. After a brief introduction to the technique, the materials and methods sections describe two different methods to obtain SCN slices — the first one for fixed tissue, the second one for fresh frozen tissue — followed by the description of two methods of antibody detection: the indirect method and the avidin-biotin complex one. In addition, some remedies to the most common problems encountered while performing ICC and some alternative protocols are discussed.
Mesh Headings (Keywords): Animals, Antibodies, Cryoultramicrotomy, Immunohistochemistry, Suprachiasmatic Nucleus, Tissue Fixation
Check for Full Text / PubMed Unique Identifier (PMID): 17417040
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