A Reversible Form of Lysine Acetylation in the Er and Golgi Lumen Controls the Molecular Stabilization of Bace1.
From: Department of Medicine, University of Madison-Wisconsin, Madison, WI 53705, USA.
The Biochemical journal
- Publish Date: Nov 2007
- ISSN: 1470-8728
- Volume: 407
- Issue: 3
- Pages: 383-95
- Medium: Internet
- Language: English
- Citation (JAMA): Costantini Claudio, Ko Mi Hee, Jonas Mary Cabell, et al. A Reversible Form of Lysine Acetylation in the Er and Golgi Lumen Controls the Molecular Stabilization of Bace1.. Biochem. J. Nov 2007;407:383-95
Abstract
The lipid second messenger ceramide regulates the rate of beta cleavage of the Alzheimer’s disease APP (amyloid precursor protein) by affecting the molecular stability of the beta secretase BACE1 (beta-site APP cleaving enzyme 1). Such an event is stimulated in the brain by the normal process of aging, and is under the control of the general aging programme mediated by the insulin-like growth factor 1 receptor. In the present study we report that BACE1 is acetylated on seven lysine residues of the N-terminal portion of the nascent protein. This process involves lysine acetylation in the lumen of the ER (endoplasmic reticulum) and is followed by deacetylation in the lumen of the Golgi apparatus, once the protein is fully mature. We also show that specific enzymatic activities acetylate (in the ER) and deacetylate (in the Golgi apparatus) the lysine residues. This process requires carrier-mediated translocation of acetyl-CoA into the ER lumen and is stimulated by ceramide. Site-directed mutagenesis indicates that lysine acetylation is necessary for nascent BACE1 to leave the ER and move ahead in the secretory pathway, and for the molecular stabilization of the protein.
Mesh Headings (Keywords): Acetylation, Amino Acid Sequence, Amyloid Precursor Protein Secretases, Animals, Aspartic Endopeptidases, CHO Cells, Cricetinae, Cricetulus, Endoplasmic Reticulum, Enzyme Stability, Golgi Apparatus, Humans, Lysine, Molecular Sequence Data, Mutagenesis, Site-Directed, Protein Transport
Check for Full Text / PubMed Unique Identifier (PMID): 17425515
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