Stimulation of Fission Yeast and Mouse Hop2-mnd1 of the Dmc1 and Rad51 Recombinases.
From: Genome Stability Laboratory, Laval University Cancer Research Center, Hôtel-Dieu de Québec, 9 McMahon, Quebec city, QC, Canada G1R 2J6.
Nucleic acids research
- Publish Date: 2007
- ISSN: 1362-4962
- Volume: 35
- Issue: 8
- Pages: 2719-33
- Medium: Internet
- Language: English
- Citation (JAMA): Ploquin Mickaël, Petukhova Galina V, Morneau Dany, et al. Stimulation of Fission Yeast and Mouse Hop2-mnd1 of the Dmc1 and Rad51 Recombinases.. Nucleic Acids Res. 2007;35:2719-33
Abstract
Genetic analysis of fission yeast suggests a role for the spHop2-Mnd1 proteins in the Rad51 and Dmc1-dependent meiotic recombination pathways. In order to gain biochemical insights into this process, we purified Schizosaccharomyces pombe Hop2-Mnd1 to homogeneity. spHop2 and spMnd1 interact by co-immunoprecipitation and two-hybrid analysis. Electron microscopy reveals that S. pombe Hop2-Mnd1 binds single-strand DNA ends of 3’-tailed DNA. Interestingly, spHop2-Mnd1 promotes the renaturation of complementary single-strand DNA and catalyses strand exchange reactions with short oligonucleotides. Importantly, we show that spHop2-Mnd1 stimulates spDmc1-dependent strand exchange and strand invasion. Ca(2+) alleviate the requirement for the order of addition of the proteins on DNA. We also demonstrate that while spHop2-Mnd1 affects spDmc1 specifically, mHop2 or mHop2-Mnd1 stimulates both the hRad51 and hDmc1 recombinases in strand exchange assays. Thus, our results suggest a crucial role for S. pombe and mouse Hop2-Mnd1 in homologous pairing and strand exchange and reveal evolutionary divergence in their specificity for the Dmc1 and Rad51 recombinases.
Mesh Headings (Keywords): Animals, Cell Cycle Proteins, Chromatography, Gel, DNA, Single-Stranded, DNA-Binding Proteins, Mice, Rad51 Recombinase, Recombinases, Recombination, Genetic, Schizosaccharomyces pombe Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 17426123
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