A Choice Behavior for Morphine Reveals Experience-dependent Drug Preference and Underlying Neural Substrates in Developing Larval Zebrafish.
From: Department of Biopharmaceutical Sciences, University of California, San Francisco, CA 94143-2811, USA.
Neuroscience
- Publish Date: May 2007
- ISSN: 0306-4522
- Volume: 146
- Issue: 3
- Pages: 1109-16
- Medium: Print
- Language: English
- Citation (JAMA): Bretaud S, Li Q, Lockwood B L, et al. A Choice Behavior for Morphine Reveals Experience-dependent Drug Preference and Underlying Neural Substrates in Developing Larval Zebrafish.. Neuroscience May 2007;146:1109-16
Abstract
Transparent larval zebrafish offer the opportunity to unravel genetic and neuronal networks underlying behavior in a developing system. In this study, we developed a choice chamber paradigm to measure reward-associated behavior in larval zebrafish. In the chamber where larval zebrafish have a choice of spending their time in either a water- or morphine-containing compartment, larvae that have previously experienced morphine spend significantly more time in the compartment containing morphine. This behavior can be attentuated by pre-treatment with antagonists of the opioid receptor or the dopamine receptor, and furthermore, is impaired in the too few mutant, which has a genetic deficiency in the production of specific groups of dopaminergic and serotonergic neurons in the ventral forebrain. These results uncover a choice behavior for an addictive substance in larval zebrafish that is mediated through central opioid and monoaminergic neurotransmitter systems.
Mesh Headings (Keywords): Analgesics, Opioid, Animals, Biogenic Amines, Choice Behavior, Chromatography, Liquid, Cloning, Molecular, DNA Mutational Analysis, Dopamine, Immunohistochemistry, In Situ Hybridization, Larva, Mass Spectrometry, Morphine, Motor Activity, Naloxone, Narcotic Antagonists, Nerve Net, Neurotransmitter Agents, Receptors, Odorant, Receptors, Opioid, Receptors, Opioid, mu, Reinforcement (Psychology), Reverse Transcriptase Polymerase Chain Reaction, Reward, Signal Transduction, Zebrafish
Check for Full Text / PubMed Unique Identifier (PMID): 17428610
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