Glutamatergic and Purinergic Receptor-mediated Calcium Transients in Bergmann Glial Cells.
From: Vollum Institute, Oregon Health & Science University, Portland, Oregon 97239, USA.
The Journal of neuroscience : the official journal of the Society for Neuroscience
- Publish Date: Apr 2007
- ISSN: 1529-2401
- Volume: 27
- Issue: 15
- Pages: 4027-35
- Medium: Internet
- Language: English
- Citation (JAMA): Piet Richard, Jahr Craig E, et al. Glutamatergic and Purinergic Receptor-mediated Calcium Transients in Bergmann Glial Cells.. J. Neurosci. Apr 2007;27:4027-35
Abstract
Astrocytes respond to neuronal activity with [Ca2+]i increases after activation of specific receptors. Bergmann glial cells (BGs), astrocytes of the cerebellar molecular layer (ML), express various receptors that can mobilize internal Ca2+. BGs also express Ca2+ permeable AMPA receptors that may be important for maintaining the extensive coverage of Purkinje cell (PC) excitatory synapses by BG processes. Here, we examined Ca2+ signals in single BGs evoked by synaptic activity in cerebellar slices. Short bursts of high-frequency stimulation of the ML elicited Ca2+ transients composed of a small-amplitude fast rising phase, followed by a larger and slower rising phase. The first phase resulted from Ca2+ influx through AMPA receptors, whereas the second phase required release of Ca2+ from internal stores initiated by P2 purinergic receptor activation. We found that such Ca2+ responses could be evoked by direct activation of neurons releasing ATP onto BGs or after activation of metabotropic glutamate receptor 1 on these neurons. Moreover, examination of BG and PC responses to various synaptic stimulation protocols suggested that ML interneurons are likely the cellular source of ATP.
Mesh Headings (Keywords): Animals, Calcium Signaling, Cerebellum, Neuroglia, Rats, Receptors, Glutamate, Receptors, Purinergic
Check for Full Text / PubMed Unique Identifier (PMID): 17428980
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.