Cpg Hypomethylation in a Large Domain Encompassing the Embryonic Beta-like Globin Genes in Primitive Erythrocytes.
From: Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire, USA.
Molecular and cellular biology
- Publish Date: Jul 2007
- ISSN: 0270-7306
- Volume: 27
- Issue: 13
- Pages: 5047-54
- Medium: Print
- Language: English
- Citation (JAMA): Hsu Mei, Mabaera Rodwell, Lowrey Christopher H, et al. Cpg Hypomethylation in a Large Domain Encompassing the Embryonic Beta-like Globin Genes in Primitive Erythrocytes.. Mol. Cell. Biol. Jul 2007;27:5047-54
Abstract
There is little evidence addressing the role of CpG methylation in transcriptional control of genes that do not contain CpG islands. This is reflected in the ongoing debate about whether CpG methylation merely suppresses retroelements or if it also plays a role in developmental and tissue-specific gene regulation. The genes of the beta-globin locus are an important model of mammalian developmental gene regulation and do not contain CpG islands. We have analyzed the methylation status of regions in the murine beta-like globin locus in uncultured primitive and definitive erythroblasts and other cultured primary and transformed cell types. A large ( approximately 20-kb) domain is hypomethylated only in primitive erythroid cells; it extends from the region just past the locus control region to before beta-major and encompasses the embryonic genes Ey, beta h1, and beta h0. Even retrotransposons in this region are hypomethylated in primitive erythroid cells. The existence of this large developmentally regulated domain of hypomethylation supports a mechanistic role for DNA methylation in developmental regulation of globin genes.
Mesh Headings (Keywords): Animals, Cells, Cultured, CpG Islands, DNA Methylation, Embryo, Mammalian, Erythrocytes, Gene Expression Regulation, Globins, Locus Control Region, Mice, Models, Genetic, Promoter Regions (Genetics), RNA, Messenger, Regulatory Sequences, Nucleic Acid, Repetitive Sequences, Nucleic Acid, Transcription, Genetic
Check for Full Text / PubMed Unique Identifier (PMID): 17452448
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