Raised Serum, Adipocyte, and Adipose Tissue Retinol-binding Protein 4 in Overweight Women with Polycystic Ovary Syndrome: Effects of Gonadal and Adrenal Steroids.
From: Endocrinology and Metabolism Group, Clinical Sciences Research Institute, Warwick Medical School, University of Warwick, Coventry CV4 7AL, United Kingdom.
The Journal of clinical endocrinology and metabolism
- Publish Date: Jul 2007
- ISSN: 0021-972X
- Volume: 92
- Issue: 7
- Pages: 2764-72
- Medium: Print
- Language: English
- Citation (JAMA): Tan Bee K, Chen Jing, Lehnert Hendrik, et al. Raised Serum, Adipocyte, and Adipose Tissue Retinol-binding Protein 4 in Overweight Women with Polycystic Ovary Syndrome: Effects of Gonadal and Adrenal Steroids.. J. Clin. Endocrinol. Metab. Jul 2007;92:2764-72
Abstract
CONTEXT: Polycystic ovary syndrome (PCOS) is associated with insulin resistance and obesity. Recent studies have shown that serum retinol-binding protein 4 (RBP4) levels increase with obesity. Currently, no data exist on the relative expression of RBP4 in either serum or adipose tissue of PCOS women. OBJECTIVES: mRNA expression of RBP4 from sc and omental (om) adipose tissue and sc adipocytes in overweight PCOS women were compared with matched controls; RBP4 protein in adipose tissue and serum RBP4 levels were also assessed. Additionally, we studied the effects of testosterone, 17beta-estradiol, androstenedione, and dehydroepiandrosterone sulfate on RBP4 expression in adipose tissue explants. DESIGN: Real-time RT-PCR and Western blotting were used to assess the relative mRNA and protein expression of RBP4. Biochemical measurements were also performed. RESULTS: Compared with controls, there was significant up-regulation of RBP4 mRNA in sc (P < 0.05) and om (P < 0.01) adipose tissue as well as isolated sc adipocytes (P < 0.01) of PCOS women. In addition to elevated serum RBP4 levels in PCOS women (P < 0.05), RBP4 protein levels were significantly greater in sc and om adipose tissue of PCOS women (P < 0.05 and P < 0.05, respectively). Furthermore, in human sc and om adipose tissue explants, 17beta-estradiol significantly increased RBP4 mRNA expression, protein levels, and secretion into the culture media (P < 0.05). CONCLUSIONS: The precise reason for elevated levels of RBP4 in overweight PCOS women is unknown, but it appears that 17beta-estradiol may play a role in their regulation in adipose tissue.
Mesh Headings (Keywords): Adipocytes, Adult, Androgens, Androstenedione, Cells, Cultured, Dehydroepiandrosterone Sulfate, Estradiol, Female, Gene Expression, Glucose Transporter Type 4, Humans, Hypoglycemic Agents, Insulin, Omentum, Overweight, Polycystic Ovary Syndrome, RNA, Messenger, Retinol-Binding Proteins, Retinol-Binding Proteins, Plasma, Steroids, Subcutaneous Fat, Testosterone
Check for Full Text / PubMed Unique Identifier (PMID): 17456573
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