Cns Progenitor Cells Promote a Permissive Environment for Neurite Outgrowth Via a Matrix Metalloproteinase-2-dependent Mechanism.
From: Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114, USA.
The Journal of neuroscience : the official journal of the Society for Neuroscience
- Publish Date: Apr 2007
- ISSN: 1529-2401
- Volume: 27
- Issue: 17
- Pages: 4499-506
- Medium: Internet
- Language: English
- Citation (JAMA): Zhang Yiqin, Klassen Henry J, Tucker Budd A, et al. Cns Progenitor Cells Promote a Permissive Environment for Neurite Outgrowth Via a Matrix Metalloproteinase-2-dependent Mechanism.. J. Neurosci. Apr 2007;27:4499-506
Abstract
Transplantation of progenitor cells to the CNS has shown promise in neuronal and glial replacement and as a means of rescuing host neurons from apoptosis. Here we examined the effect of progenitor grafts on neurite extension in the degenerating retina of rd1 (retinal degeneration 1) mice. Transplantation of retinal progenitor cells induced increased matrix metalloproteinase-2 (MMP2) secretion, partly from activated glial cells, which was then activated by neuronally expressed MMP14. Active MMP2 resulted in proteolysis of the neurite outgrowth inhibitors CD44 and neurocan in the degenerative retina, allowing significantly increased neurite outgrowth across the border between abutting nondystrophic and rd1 retinas. Progenitor-induced enhancement of outgrowth was abrogated by an MMP inhibitor or by coculture with retinal explants from MMP2-/- mice. This study provides the first identification of an MMP2-dependent mechanism by which exogenous progenitor cells alter the host environment to promote neural regeneration. This suggests a novel therapeutic role for progenitor cells in the treatment of CNS degenerative diseases.
Mesh Headings (Keywords): Animals, Antigens, CD44, Cell Movement, Green Fluorescent Proteins, Matrix Metalloproteinase 2, Mice, Mice, Inbred C3H, Mice, Transgenic, Nerve Regeneration, Nerve Tissue Proteins, Neurites, Neurons, Proteoglycans, Retina, Retinal Diseases, Stem Cell Transplantation, Stem Cells
Check for Full Text / PubMed Unique Identifier (PMID): 17460063
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.