Calcitonin Gene-related Peptide Induces the Expression of Acetylcholinesterase-associated Collagen Colq in Muscle: a Distinction in Driving Two Different Promoters Between Fast- and Slow-twitch Muscle Fibers.
From: Departments of Biology and Molecular Neuroscience Center, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, China.
Journal of neurochemistry
- Publish Date: Aug 2007
- ISSN: 0022-3042
- Volume: 102
- Issue: 4
- Pages: 1316-28
- Medium: Print
- Language: English
- Citation (JAMA): Choi Roy C Y, Ting Annie K L, Lau Faye T C, et al. Calcitonin Gene-related Peptide Induces the Expression of Acetylcholinesterase-associated Collagen Colq in Muscle: a Distinction in Driving Two Different Promoters Between Fast- and Slow-twitch Muscle Fibers.. J. Neurochem. Aug 2007;102:1316-28
Abstract
The presence of a collagenous protein (ColQ) characterizes the collagen-tailed forms of acetylcholinesterase at vertebrate neuromuscular junctions (nmjs). Two ColQ transcripts as ColQ-1 and ColQ-1a, driven by two promoters: pColQ-1 and pColQ-1a, were found in mammalian slow- and fast-twitch muscles, respectively, which have distinct expression pattern in different muscle fibers. In this study, we show the differential expression of CoQ in different muscles is triggered by calcitonin gene-related peptide (CGRP), a known motor neuron-derived factor. Application of CGRP, or dibutyryl-cAMP (Bt(2)-cAMP), in cultured myotubes induced the expression of ColQ-1a transcript and promoter activity; however, the expression of ColQ-1 transcript did not respond to CGRP or Bt(2)-cAMP. The CGRP-induced gene activation was blocked by an adenylyl cyclase inhibitor or a dominant negative mutant of cAMP-responsive element (CRE) binding protein (CREB). Two CRE sites were mapped within the ColQ-1a promoter, and mutations of the CRE sites abolished the response of CGRP or Bt(2)-cAMP. In parallel, CGRP receptor complex was dominantly expressed at the nmjs of fast muscle but not of slow muscle. These results suggested that the expression of ColQ-1a at the nmjs of fast-twitch muscle was governed by a CGRP-mediated cAMP signaling mechanism.
Mesh Headings (Keywords): Acetylcholinesterase, Animals, Bucladesine, CREB-Binding Protein, Calcitonin Gene-Related Peptide, Cells, Cultured, Chick Embryo, Chickens, Chromatin Immunoprecipitation, Dose-Response Relationship, Drug, Electrophoretic Mobility Shift Assay, Gene Expression Regulation, Green Fluorescent Proteins, Humans, Muscle Cells, Muscle Fibers, Fast-Twitch, Muscle Fibers, Slow-Twitch, Promoter Regions (Genetics), Receptors, Calcitonin Gene-Related Peptide, Receptors, Cholinergic, Time Factors, Transfection
Check for Full Text / PubMed Unique Identifier (PMID): 17488278
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