Vaccinia Virus Temperature-sensitive Mutants in the A28 Gene Produce Non-infectious Virions That Bind to Cells but Are Defective in Entry.
From: Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, FL 32610-0266, USA. pturner@mgm.ufl.edu
Virology
- Publish Date: Sep 2007
- ISSN: 0042-6822
- Volume: 366
- Issue: 1
- Pages: 62-72
- Medium: Print
- Language: English
- Citation (JAMA): Turner Peter C, Dilling Bradley P, Prins Cindy, et al. Vaccinia Virus Temperature-sensitive Mutants in the A28 Gene Produce Non-infectious Virions That Bind to Cells but Are Defective in Entry.. Virology Sep 2007;366:62-72
Abstract
The vaccinia virus temperature-sensitive mutations Cts6 and Cts9 were mapped by marker rescue and DNA sequencing to the A28 gene. Cts6 and Cts9 contain an identical 2-bp deletion truncating the A28 protein and removing the fourth conserved cysteine near the C-terminus. Cts9 mutant virions produced at 40 degrees C were non-infectious and unable to cause cytopathic effect. However, the mutant A28 protein localized to purified mature virions (MV) at 31 degrees C and 40 degrees C. MV of Cts9 produced at 40 degrees C bound to cells but did not enter cells. Low pH treatment of Cts9-infected cells at 18 h p.i. failed to produce fusion from within at 40 degrees C, but gave fusion at 31 degrees C. Adsorption of Cts9 mutant virions to cells followed by low pH treatment showed a defect in fusion from without. The Cts9 phenotype suggests that the A28 protein is involved in both virus entry and cell-cell fusion, and supports the linkage between the two processes.
Mesh Headings (Keywords): 3’ Untranslated Regions, Animals, Cell Fusion, Cell Line, Cercopithecus aethiops, DNA Viruses, Genes, Viral, Genome, Viral, Haplorhini, Mutation, Recombination, Genetic, Temperature, Vaccinia virus, Viral Vaccines, Virion
Check for Full Text / PubMed Unique Identifier (PMID): 17499330
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