Human Cd4+cd25+ Regulatory T Cells: Proteome Analysis Identifies Galectin-10 As a Novel Marker Essential for Their Anergy and Suppressive Function.
From: Department of Dermatology, Johannes Gutenberg-University, Mainz, Germany.
Blood
- Publish Date: Sep 2007
- ISSN: 0006-4971
- Volume: 110
- Issue: 5
- Pages: 1550-8
- Medium: Print
- Language: English
- Citation (JAMA): Kubach Jan, Lutter Petra, Bopp Tobias, et al. Human Cd4+cd25+ Regulatory T Cells: Proteome Analysis Identifies Galectin-10 As a Novel Marker Essential for Their Anergy and Suppressive Function.. Blood Sep 2007;110:1550-8
Abstract
CD4(+)CD25(+)Foxp3(+) regulatory T cells (CD25(+) Treg cells) direct the maintenance of immunological self-tolerance by active suppression of autoaggressive T-cell populations. However, the molecules mediating the anergic state and regulatory function of CD25(+) Treg cells are still elusive. Using differential proteomics, we identified galectin-10, a member of the lectin family, as constitutively expressed in human CD25(+) Treg cells, while they are nearly absent in resting and activated CD4(+) T cells. These data were confirmed on the mRNA and protein levels. Single-cell staining and flow cytometry showed a strictly intracellular expression of galectin-10 in CD25(+) Treg cells. Specific inhibition of galectin-10 restored the proliferative capacity of CD25(+) Treg cells and abrogated their suppressive function. Notably, first identified here as expressed in human T lymphocytes, galectin-10 is essential for the functional properties of CD25(+) Treg cells.
Mesh Headings (Keywords): Antibodies, Monoclonal, Biological Markers, Cell Proliferation, Cells, Cultured, Clonal Anergy, Forkhead Transcription Factors, Galectins, Gene Expression Regulation, Humans, Proteome, Self Tolerance, T-Lymphocytes, Regulatory
Check for Full Text / PubMed Unique Identifier (PMID): 17502455
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