• Kreiling Jill A
• Williams Geoffrey
• Creton Robbert

Developmental dynamics : an official publication of the American Association of Anatomists

• Publish Date: Jul 2007
• ISSN: 1058-8388
• Volume: 236
• Issue: 7
• Pages: 1963-9
• Medium: Print
• Language: English
• Citation (JAMA): Kreiling Jill A, , Williams Geoffrey, et al. Analysis of Kupffer's Vesicle in Zebrafish Embryos Using a Cave Automated Virtual Environment.. Dev. Dyn. Jul 2007;236:1963-9

Abstract

Previous studies show that cilia in Kupffer’s vesicle (KV) generate a counterclockwise flow of fluid and provide convincing evidence that this flow regulates left/right asymmetry. We hypothesized that the distribution of cilia in KV leads to this directional flow. However, there are limitations in determining the localization of structures when viewing a three-dimensional (3-D) image on a 2-D computer screen. We analyzed the distribution of KV cilia in the Cave, an immersive virtual environment that displays stacks of confocal images in 3-D. We found 80% of the cilia are located on the dorsal surface and 20% were located on the ventral surface of the vesicle. We confirmed the ventral location of some cilia by electron microscopy. There is an asymmetrical distribution of cilia on the dorsal surface, with the anterior one third containing 50% and the posterior one third containing 20% of the cilia. This dorsal-anterior patch could explain the directionality of the flow, and could drive local differences in flow rate.

This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.

Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.

The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.