Fluid Shear Stress Enhances the Sphingosine 1-phosphate Responses in Cell-cell Interactions Between Platelets and Endothelial Cells.
From: Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Biochemical and biophysical research communications
- Publish Date: Jul 2007
- ISSN: 0006-291X
- Volume: 358
- Issue: 4
- Pages: 1054-7
- Medium: Print
- Language: English
- Citation (JAMA): Aoki Shinya, Osada Makoto, Kaneko Makoto, et al. Fluid Shear Stress Enhances the Sphingosine 1-phosphate Responses in Cell-cell Interactions Between Platelets and Endothelial Cells.. Biochem. Biophys. Res. Commun. Jul 2007;358:1054-7
Abstract
Fluid shear stress modulates the functional responses of platelets and vascular cells, and plays an important role in the pathogenesis of vascular disorders, including atherosclerosis and restenosis. Since shear stress induces activation of platelets, which abundantly store sphingosine 1-phosphate (Sph-1-P), and upregulates the mRNA expression of S1P(1), the most important Sph-1-P receptor expressed on the endothelial cells, we examined the effects of shear stress on the Sph-1-P-related responses involving these cells. Shear stress was found to induce Sph-1-P release from the platelets in a shear intensity- and time-dependent manner. Inhibitors of protein kinase C suppressed this mechanical force-induced Sph-1-P release, suggesting involvement of this kinase. On the other hand, in vascular endothelial cells, shear stress increased S1P(1) protein expression, as revealed by flow-cytometric analysis, and the responsiveness to Sph-1-P, which was assessed by monitoring the intracellular Ca(2+) concentration. These results indicate that shear stress enhances the Sph-1-P responses in cell-cell interactions between platelets and endothelial cells.
Mesh Headings (Keywords): Blood Flow Velocity, Blood Platelets, Cell Communication, Cells, Cultured, Endothelial Cells, Humans, Lysophospholipids, Mechanotransduction, Cellular, Shear Strength, Sphingosine, Stress, Mechanical
Check for Full Text / PubMed Unique Identifier (PMID): 17512899
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.