17-beta Estradiol Enhances Osteogenic and Adipogenic Differentiation of Human Adipose-derived Stromal Cells.
From: Department of Orthodontics, University of Illinois at Chicago, Chicago, Illinois 60612-7211, USA. liuhong1@uic.edu
Tissue engineering
- Publish Date: Jun 2007
- ISSN: 1076-3279
- Volume: 13
- Issue: 6
- Pages: 1197-203
- Medium: Print
- Language: English
- Citation (JAMA): Hong Liu, Colpan Aylin, Peptan Ioana A, et al. 17-beta Estradiol Enhances Osteogenic and Adipogenic Differentiation of Human Adipose-derived Stromal Cells.. Tissue Eng. Jun 2007;13:1197-203
Abstract
Adipose-derived stromal cells (ASCs) possess multiple differentiation potentials and may serve as a cell source, if effectively modulated, for regenerative medicine and tissue engineering. Due to estrogen’s function in tissue and organ development through regulating cell proliferation and differentiation, we hypothesized that an estrogen supplement may effectively enhance the multiple differentiation potentials of human ASCs. 17-Beta estradiol (E2) was investigated for modulating in vitro osteogenic and adipogenic differentiation in human ASCs isolated from a healthy female donor. After ASCs’ exposure to osteogenic and adipogenic differentiation medium supplemented with different concentrations of E2, osteogenic markers (alkaline phosphatase activity, extracellular matrix, calcium deposition, and osteocalcin expression) and adipogenic parameters (lipid accumulation and differentiated cell population) significantly improved. Estrogen’s enhancement is dose dependent and linked to differing alpha and beta estrogen receptors. Our data preliminarily demonstrate that estrogen can modulate the differentiation, and potentially improve the efficiency of ASCs in stem cell-based tissue engineering and regeneration. However, further study is needed to verify the regulatory functions of estrogen on ASC differentiations of donors with different ages and genders.
Mesh Headings (Keywords): Adipocytes, Cell Culture Techniques, Cell Differentiation, Cells, Cultured, Dose-Response Relationship, Drug, Estradiol, Female, Humans, Middle Aged, Osteogenesis, Stem Cells, Tissue Engineering
Check for Full Text / PubMed Unique Identifier (PMID): 17518737
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.