Vascular Endothelial Growth Factor is Induced by the Inflammatory Cytokines Interleukin-6 and Oncostatin M in Human Adipose Tissue in Vitro and in Murine Adipose Tissue in Vivo.
From: Department of Internal Medicine II, Medical University Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria.
Arteriosclerosis, thrombosis, and vascular biology
- Publish Date: Jul 2007
- ISSN: 1524-4636
- Volume: 27
- Issue: 7
- Pages: 1587-95
- Medium: Internet
- Language: English
- Citation (JAMA): Rega G, Kaun C, Demyanets S, et al. Vascular Endothelial Growth Factor is Induced by the Inflammatory Cytokines Interleukin-6 and Oncostatin M in Human Adipose Tissue in Vitro and in Murine Adipose Tissue in Vivo.. Arterioscler. Thromb. Vasc. Biol. Jul 2007;27:1587-95
Abstract
OBJECTIVES: It is believed that adipose tissue acts as an endocrine organ by producing inflammatory mediators and thereby contributes to the increased cardiovascular risk seen in obesity. A link between adipose tissue mass and angiogenesis has been suggested. Vascular endothelial growth factor (VEGF) seems to be implicated in this process. Members of the glycoprotein (gp)130 ligand family regulate VEGF expression in other cells. METHODS AND RESULTS: We used tissue explants as well as primary cultures of preadipocytes and adipocytes from human subcutaneous and visceral adipose tissue to investigate whether the gp130 ligands oncostatin M (OSM), interleukin-6 (IL-6), leukemia inhibitory factor (LIF), and cardiotrophin-1 (CT-1) regulate VEGF expression in human adipose tissue. Human subcutaneous and visceral adipose tissue responded to treatment with IL-6 and OSM with a significant increase in VEGF production. Human preadipocytes were isolated from subcutaneous and visceral adipose tissue. Adipocyte-differentiation was induced by hormone-supplementation. All cell types responded to IL-6 and OSM with a robust increase in VEGF protein production and a similar increase in VEGF-specific mRNA. Furthermore, IL-1beta synergistically enhanced the effect of OSM on VEGF production. AG-490, a JAK/STAT inhibitor, abolished the OSM-dependent VEGF induction almost completely. In mice, IL-6 and OSM increased serum levels of VEGF and VEGF mRNA and vessel density in adipose tissue. CONCLUSION: We speculate that the inflammatory cytokines IL-6 and OSM might support angiogenesis during adipose tissue growth by upregulating VEGF.
Mesh Headings (Keywords): Adipocytes, Animals, Antigens, CD34, Cells, Cultured, Cytokine Receptor gp130, Humans, Inflammation Mediators, Interleukin-6, Mice, Models, Animal, Oncostatin M, RNA, Messenger, Sensitivity and Specificity, Up-Regulation, Vascular Endothelial Growth Factors
Check for Full Text / PubMed Unique Identifier (PMID): 17525365
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.