Enhanced Accumulation of Phosphorylated Alpha-synuclein in Double Transgenic Mice Expressing Mutant Beta-amyloid Precursor Protein and Presenilin-1.
From: Department of Neurology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
Journal of neuroscience research
- Publish Date: Aug 2007
- ISSN: 0360-4012
- Volume: 85
- Issue: 10
- Pages: 2246-52
- Medium: Print
- Language: English
- Citation (JAMA): Kurata Tomoko, Kawarabayashi Takeshi, Murakami Tetsuro, et al. Enhanced Accumulation of Phosphorylated Alpha-synuclein in Double Transgenic Mice Expressing Mutant Beta-amyloid Precursor Protein and Presenilin-1.. J. Neurosci. Res. Aug 2007;85:2246-52
Abstract
A recent report showed that the accumulation of alpha-synuclein (alpha-syn) was detected in the brains of one-third of Alzheimer’s disease and Down syndrome patients. However, the relationship between amyloid-beta protein (Abeta) and alpha-syn remains unclear. We analyzed the relation between the mutation of presenilin-1 (PS-1) and the pathological features of beta-amyloidosis and alpha-synucleinopathy. We generated doubly transgenic mice overexpressing mutant beta-amyloid precursor protein (betaAPP; Tg2576) and mutant PS-1 (PS1L286Vtg; line 198) and analyzed 19 double Tg betaAPP(+)/PS(+) mice at 5-23 months (young to old), 23 age-matched single Tg betaAPP(+)/PS(-) mice, and 11 non-Tg littermates. Immunohistochemical comparison was performed in these three groups by counting the area and the number of alpha-syn- or phosphorylated alpha-syn (palpha-syn)-positive dystrophic neurites per plaque (ASPDN, pASPDN). The acceleration of Abeta pathology was found with earlier onset and exaggerated numbers in double Tg betaAPP(+)/PS(+) compared with single Tg betaAPP(+)/PS(-) mouse brains. The accumulation of ASPDN and pASPDN was also accelerated in double Tg betaAPP(+)/PS(+) compared with single Tg betaAPP(+)/PS(-) mouse brains, especially in pASPDN. The number and area of alpha-syn and palpha-syn, and the ratio of palpha-syn positive neurites were significantly higher in double Tg betaAPP(+)/PS(+) than in single Tg betaAPP(+)/PS(-) mouse brains in middle-aged and old groups. Additional overexpression of mutant PS-1 accelerated Abeta-induced alpha-synucleinopathy and further facilitated the phosphorylation of alpha-syn, suggesting a direct association between mutant PS-1 and phosphorylation of alpha-syn.
Mesh Headings (Keywords): Amyloid beta-Protein Precursor, Amyloidosis, Animals, Brain, Cerebral Cortex, Immunohistochemistry, Mice, Mice, Transgenic, Mutation, Neurites, Phosphorylation, Presenilin-1, Time Factors, alpha-Synuclein
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