Dietary Polyunsaturated Fatty Acids Alter Myocardial Protein Kinase C Expression and Affect Cardioprotection Induced by Chronic Hypoxia.
From: Charles University, Faculty of Science, Department of Biochemistry, Hlavova 8, 128 43 Prague 2, Czech Republic.
Experimental biology and medicine (Maywood, N.J.)
- Publish Date: Jun 2007
- ISSN: 1535-3702
- Volume: 232
- Issue: 6
- Pages: 823-32
- Medium: Print
- Language: English
- Citation (JAMA): Hlavácková Markéta, Neckár Jan, Jezková Jana, et al. Dietary Polyunsaturated Fatty Acids Alter Myocardial Protein Kinase C Expression and Affect Cardioprotection Induced by Chronic Hypoxia.. Exp. Biol. Med. (Maywood) Jun 2007;232:823-32
Abstract
We examined the influence of dietary fatty acid (FA) classes on the expression of protein kinase C (PKC) delta and epsilon in relation to the cardioprotective effects of chronic intermittent hypoxia (CIH). Adult male Wistar rats were fed a nonfat diet enriched with 10% lard (saturated FA [SFA]), fish oil (n-3 polyunsaturated FA [n-3 PUFA]), or corn oil (n-6 PUFA) for 10 weeks. After 4 weeks on the diet, each group was divided into two subgroups that were either exposed to CIH in a barochamber (7000 m, 8 hrs/ day) or kept at normoxia for an additional 5-6 weeks. A FA phospholipid profile and Western blot analysis of PKC were performed in left ventricles. Infarct size was assessed in anesthetized animals subjected to 20-min coronary artery occlusion and 3-hr reperfusion. CIH decreased the n-6/n-3 PUFA ratio in all groups by 23% independently of the initial value set by various diets. The combination of n-3 diet and CIH had a stronger antiarrhythmic effect during reperfusion than the n-3 diet alone; this effect was less pronounced in rats fed the n-6 diet. The normoxic n-6 group exhibited smaller infarctions (by 22%) than the n-3 group. CIH decreased the infarct size in n-3 and SFA groups (by 20% and 23%, respectively) but not in the n-6 group. Unlike PKC epsilon, the abundance of PKC delta in the myocardial particulate fraction was increased by CIH except for the n-6 group. Myocardial infarct size was negatively correlated (r=- 0.79) with the abundance of PKC delta in the particulate fraction. We conclude that lipid diets modify the infarct size-limiting effect of CIH by a mechanism that involves the PKC delta-dependent pathway.
Mesh Headings (Keywords): Animals, Anoxia, Blood Pressure, Cardiotonic Agents, Dietary Fats, Fatty Acids, Fatty Acids, Omega-3, Fatty Acids, Omega-6, Heart Rate, Male, Myocardial Infarction, Myocardial Reperfusion Injury, Myocardium, Protein Kinase C-delta, Protein Kinase C-epsilon, Rats, Rats, Wistar
Check for Full Text / PubMed Unique Identifier (PMID): 17526775
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